FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3890848
Sequences flagged as poor quality0
Sequence length20-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT86350.096.035615
AGTATTC91550.093.0445867
GTATTCC90900.092.970328
TAGTATT93950.090.474036
TATTCCA106000.079.89819
CATAGTA41950.079.570654
GATAGTA26050.075.1960144
CGCACTA6400.074.4155042
CGTACTA4050.073.87372
CGGACTA5500.065.499532
GTCTACT26600.062.6254166
ACGATCA6900.061.702522145
GGATAGT22300.061.5827263
TATAAAG63050.060.2945635
GCATAGT21900.057.6909263
TAAAGAC67600.057.679067
CGGGATA7700.057.1263471
CCGGTTT9600.056.002331
AAGACAC70400.055.1235669
CCGGTAA6450.054.9367521
Produced by FastQC (version 0.11.5)