FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14273631
Sequences flagged as poor quality0
Sequence length28-121
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT628670.4404415386666504No Hit
CTGCATCAAAAGAAAGGTTCAACTCTGTTAGTTGAGGACACACATCACAA228960.16040767762596636No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA145600.10200627997178854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC129450.090.143721
CTGCTGC142550.089.930961
GCATAGT249300.089.607563
CTGCAGC132950.089.575261
CTGCATA353300.089.40951
AGCTGCC38400.089.030182
CAGCAGC150250.087.753731
CATAGTA241350.086.6401144
TGCATAG275300.084.907932
TGCAGCC49700.084.586092
ATAGTAT241400.084.181865
AGCAGCC41350.082.151122
TGCTGCC43300.081.8514562
CTGCTAT277050.081.541851
GCTATAA175150.078.6676253
TGCTATA187850.077.9933242
GCTGCTA18050.077.634883
GTATTCC260150.077.506748
GCTGCTT24950.076.9259953
AGCTGCA47050.076.834262
Produced by FastQC (version 0.11.5)