FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14273631
Sequences flagged as poor quality0
Sequence length28-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT509120.35668569546179246No Hit
CTGCATCAAAAGAAAGGTTCAACTCTGTTAGTTGAGGACACACATCACAA154740.10840969617331427No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA148170.10380680290810376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC148800.097.212831
AGCTGCC41850.093.511872
CAGCAGC168450.092.230021
CTGCTGC129150.089.863371
AGCAGCC45900.089.169632
CTGCAGC120900.088.85381
GCATAGT203800.086.520733
CTGCATA277250.085.905261
CTGCTAT277600.083.9590151
CATAGTA195050.083.244654
TGCTGCC37800.082.617152
TGCATAG218200.082.070482
GCAGCAA40750.081.726443
TGCTATA196550.081.583022
GCTATAA190700.081.5272753
AGCAGCA79150.080.895062
AGCTGCT50750.080.6479952
TGCAGCC42700.080.6208342
ATAGTAT196200.080.155735
AGCACTA180050.078.752172
Produced by FastQC (version 0.11.5)