FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4754270
Sequences flagged as poor quality0
Sequence length20-137
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT246690.5188809217818929No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC74450.15659607047980026No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG65110.13695057285345597No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA58710.12348898989750266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT99150.096.891273
CTGCATA133800.095.2592851
CATAGTA95950.093.74854
ATAGTAT95200.091.579835
CTGCTAT116850.091.25171
TGCATAG111050.089.8866652
TGCTATA79350.089.802372
GCTATAA76600.088.3552553
GTATTCC101700.086.6920558
TAGTATT104850.085.462456
CAGCTGC55300.084.5137941
AGTATTC105650.084.4876257
CTGCTGC61900.083.8918151
CTGCAGC52450.079.205391
AGCACTA58500.078.904522
CAGCAGC66900.078.05311
TATTCCA119400.074.372299
CGTATAA12100.073.626144114-115
TACGTGT30450.073.60942114-115
CTGCAGT159800.073.477671
Produced by FastQC (version 0.11.5)