FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005322517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14473217
Sequences flagged as poor quality0
Sequence length5-121
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAGGGGGGACTGACACCGGGCCGCCGGGGTCCCTCCGGCCGGCCTGA1305740.9021767586294049No Hit
CAGCACCAGGCACTGCAGAGTGACCACTAACCTTCGCTCCATACCTAAGG1069910.7392344079412339No Hit
CAGCCCGGGCGAGTCGCCTTCCCTCTCCCCTGGCCTCTCCTTCCCGCCTC1043760.7211665519835707No Hit
CAGCTTTTAGCCATGCCATGCTCTATAAATCACTTCCCTATCTCAGGTAG850100.5873607781877381No Hit
CAGCCCGGGGTAGGTCCCCTGGACCTGCCGCTGGCGGACTACGCCTTCGT844560.5835330182640114No Hit
CAGCGGTGGCGGCGGCCCCGCGGACAGACGTCGAGGCATACTGCCGGTCC706490.48813612067033885No Hit
CAGCTTCTCTGTCATTCGATCCCAGGTACGATAGGGCAAGGGAGGAGCCC684000.47259707361535447No Hit
CAGCTGGCACTAAGGGCTCCTGCTGGTCAGCAGGGGCCGACAAAGCAAAT650480.44943705328262545No Hit
CAGCGGCTTGACGCTCAAGGGCGGCCTGTAACTTAAGTGATGGTCTCCCT632690.43714538378026113No Hit
CAGCTGGATGTACAGCTACACCGATCACCAGACGACTCCCACATTCGTAC627900.43383582240216534No Hit
CAGCTTAAAGGTGCCGGCGGAAAAGGTCACGTGACACCTACGGCCACCTG602690.41641744195502634No Hit
CAGCTCGTTGGAGAGGACCCGGCGAAGGGCGGGCCAGATTTGGAACTCGA595090.4111663633593002No Hit
CAGCACCTGATCCGTGAAATCACCCCCATCGTGACGCATCGAGAGGCAAA572490.39555131385095654No Hit
CAGCTCCTGGCAGTCATTCATTCGGATAACGCCGAGTAGAAGCCGACGCA564630.390120593092745No Hit
CAGCCGCCAAGTGGATCCGGGGCATCTGCGAGGCCGTGCGGGGTCTTTCC552280.38158759037469003No Hit
CAGCGGCTAGGTTCAGCCAGGCACCCGCGCCCCCAGCTAAGGCCAGGGCT542910.37511356321127504No Hit
CAGCACTTGAATCTGACTCCGATTGCGTAGCTCTAGCAGGGTAAAGAGCC529590.3659103570408707No Hit
CTGCGCTCCTCCACCTCAAACACCAGGCCGGGGTGCTCTGCAAACATCTC520880.3598923445976109No Hit
CTGCTGTCTGGCTTACGTAAACGCGCTGGACTGAGAAGGTGGCCTAGCAA491020.33926113316756046No Hit
CAGCCCACCCACACTTCCCGAGGATGCTACCCTTCTAATCAAATGGTTGG489460.33818328019264826No Hit
CAGCACCTCCCGTCCGGTCACGTCTCATGTTGGCATCATCGATCCCGGCT480090.33170925302923326No Hit
CAGCTTTGGCAGACCACCTGTGCTGGAAAGAGAGGCTGGAAGATGAGGCC471630.325863973434517No Hit
CAGCCATGGCCCCCTCCTGGATGAAGAGCGAGGTATGCGGTGAGAGAATG464430.3208892673964606No Hit
CAGCGTGGAGTTGTAGCGCCGCTGATTCTGGAGGATTAGCCGGAGTTCCC460790.3183742771216655No Hit
CTGCGCACCAGTAGGTTGGCTAGCGTGCTGGCCCGCGCGGCATCTTCCTC444540.30714664196632996No Hit
CTGCAGGGCAGGGTCAGCCCACGGCCGCGGACATCAAGGCCATGGCCGTG440660.3044658281569329No Hit
CAGCGGGGGGCTCATTGCTATGCTCGCCGATGCCGCCGAGAAGGACCTAT435030.3005758844077305No Hit
CAGCGGGGAGTGACCATCACTATGCCGGTGGCCTCCGACTACCTCCCGGA434030.299884953013556No Hit
CAGCAGTAATAGATGAAAACAAAGCTCAGCATGAAACAGCGTGGAGGCTC394740.2727382585364401No Hit
CAGCCACTCGCGGGTCCCCGTAAAACATATGGAAAGGAATGGCGTGAAAG383040.2646543612245985No Hit
CTGCTTGTCTCCGCAAAACCCGGCGGAGAGAACGTTAAGGTTTCAGGTAC373220.25786941493380494No Hit
CAGCGCTACCTGCGTTTCCAAACGGCCTTTGATTACAACCAGGAGGACCT370330.25587262320464066No Hit
CTGCTGCTATCTTTAGAGGGGAAAAGAGGAATAAGCCCCCAGACAGGGGA355110.24535664738530488No Hit
CTGCGGAGTTGAGCCCGACGAACTCCTCCAGCAGTGGCGAGTTGAGTGTC351590.24292456887781066No Hit
CTGCAGGCCCAGCCCCCCGACGAAGCATACTCCACCGCCACCCCCGGCGC346510.2394146373954042No Hit
CAGCGCCTCAGACTCCCAAGAGGAAAAAGGGATTGGGCAAAGATAGCCCC337910.23347262740550356No Hit
CAGCGAGGCCACCACGCTGGAAAGTGACTCGGGGCGGTGAAAGAGACCAC335810.23202167147773714No Hit
CAGCACCGTCAAGGTGACGGTGCCCCAGGAGGCCTTTGTGGATCTGGACG329800.22786917379874838No Hit
CAGCGCCTCTACCAGAACATTTTCATGTGCTACCGCACGCTGGAGCATGC329290.22751679878771938No Hit
CAGCGCCTCAGCCATTGCCAGCCTGGTTCCCTCCCTCAGATCCAACGACA318150.21981982305661554No Hit
CTGCACCCCCTTTATGATATTTACATTGGCAGGGATGCCGGGGAGAGGGC304030.21006387177087169No Hit
CAGCTGATCATCCTCTGTGTCAGTGGTGGAGGACAGAGGGAGGCGGCGGC298240.20606337899860133No Hit
CTGCGTCCCAAGATCGTGGGCTGTCTGCGCCGCATCTACCGCGTTAACAT298090.20595973928947517No Hit
CTGCCCTGACGGCCATCGTGAGCGAGTACGTGGATCGGACGCTACCCGAA288040.1990158787780215No Hit
CAGCTCCTTCTTGGGGTCCTTGCGCTGTGAGTTCTGCTCCAGATGGGCCA285630.19735073411806095No Hit
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT283210.1956786801441587No Hit
CTGCACAGGGCCTAAAGAAAAAGGTCTCCAACACGGTGGTCAAGTGTCTG282560.19522957473794528No Hit
CAGCTATGGACCACCAATGTGATCGTGGTGGACGAAGCTGGAACGCTTTC282190.1949739301221007No Hit
CAGCCGTGACTAGCACGAACTGGGCCTCGTGGGAGCCAAAGGTGCTAATG281270.19433827323946018No Hit
CTGCCCTCTTCGATGATGCGCCGGTACGCGGCCGCCAGGGCCCGGGTCTC276670.1911599888262575No Hit
CAGCCAGGCGTTGAAGAACCAGTAGCAAAAGACCACGGCTGTTAGAATGT266980.18446486361670664No Hit
CTGCAGCACCTCGGCGCACAGGTTGGCGGCGTTCATGGCCTCGCCCTGCA264660.1828619027822218No Hit
CAGCTTTACATAGAGGGTCTCCGTCATGTCATCAATGTTGGTCACCTTCA260830.18021563554253348No Hit
CTGCCTTGTCCGGCCATCTTTTAGACACGGAAGACAATGTGCCGCCATGG259500.17929669678828142No Hit
CTGCCACCCGGTTTAATAATTCTGAGTCCGGGCACCGTCCCTGGCCCCCT256630.17731372368700063No Hit
CAGCCATATGTGGACAGATGGACCTGAGCCACCCGCCCCCTCGTGGCCAT255290.17638787561880678No Hit
CAGCAGCTGGCCCCGAATCCCGCCGACAAGCGGACAGAGCAGACTGTTTT247080.1707153288726342No Hit
CTGCCCGGCCAGGGCCATCTCCACCAGGCAGGTCACCAGGCCCCCGTCGC245500.1696236572698385No Hit
CAGCTGGGGCCAGGGGAGTCGGCAGGTTACCCACCATTAGCCGGTGCACA243120.16797924055170319No Hit
CAGCCCCTTCATCCTTTTGAAGGAGGCCTGCAACGCCCACTTTTGGCGCG238240.16460749534813165No Hit
CAGCAGACATTCATCATTTAGAAATGTATCCAAGATTTCATTAAGTTCGG237640.16419293651162695No Hit
CAGCCCTGCGCGCCAGACGGCCGCCGCACTCAGCGAGGAGGCCTGCGCGT233530.16135320848156978No Hit
CAGCTTCGGCCTCGGCCATCTGGAGGCGGCCCTGGACGTGCTGGCATCCT233070.16103538004024953No Hit
CTGCTTGTGATCATGGAAACCACTCAGACTCTCCGCTTTAAGACCAAGGC230830.15948769371729865No Hit
CAGCACTCCTCCCTTGAGATGAGTCTGGGCATGGTCATAGCACTTGGACA226080.1562057695949698No Hit
CAGCAACGACGGGGAAGACGCGCGCCGCCTCACCCGCCACCGCATCGCCG219250.151486708172758No Hit
CTGCTTCGGGAATACGTCTCTGCGCCCAAAGCCCTGATGGAAGACCTGGC213540.14754148991202165No Hit
CTGCTCCTACAACAAATCTGGCCCCCGACCCGAAAGGAGATTAACCCTCG211630.14622181094914835No Hit
CAGCTGAGACTCCATGGAGCGGATCTTCTTTAGATAGAGCTCCCTCTCCT209850.14499195306751775No Hit
CTGCTGCCCCCTCCGGCCCGGCGTCTAAATATTTCCCGGGGGTCCGAATT207350.14326462458208153No Hit
CAGCCCGCACACCGCTCACCAGGTGGCAATTCTACCTCTAAACAGGAGGA205270.14182748728219857No Hit
CAGCTCCTGTATTTAACTTTGCGGACAGAGGCCAGAGCCGTTTGCTCCAT205080.1416962103173054No Hit
CAGCTTGGCCAGACCCTCGGTGGCCACCTGGCGCAGGTACTGCTCCTTGC204510.14130237942262594No Hit
CTGCAGAAGCTCATGGGCCTAACGGCCTGCCTGCGCCGCATGCGTCACAA203880.14086709264429603No Hit
CAGCGGTTGGGCCGAGGCGAGGAGGAGGCGTCCCGGGAGAGCCCGGAGGT199990.13817936952095722No Hit
CAGCACCTCAGCTGTTCCCAGTCTCCGACATAACCCAGAATCAACAGACT196190.13555383022309414No Hit
CAGCTGGGGCTTCTTAGGCCAGAGCGCCGAGTCCGAGGCCAGCCTGGGCG194200.1341788767486869No Hit
CTGCTGAACCTGCTGGTCAAGCTCTCGGAGAACAAGACCATCAGCGGGGT194150.1341443301789782No Hit
CAGCATCCCTGATATTCCTTGCGGTATATGGTGTGATGTCGTGTGGAGGC193660.13380577379583267No Hit
CAGCAGACGGCGGATATGGGAATTTCAGAATGAGGTGGCGGATTCAGGCG186390.1287827025601841No Hit
CTGCCCCTGGAGAACACATGCGCCCCGGAGCATCTCTCCACTTCCAGAGA186040.12854087657222302No Hit
CTGCCCACCTCCCGGTGCCCAGGGTCATCACATACACCGACTGCCCGGGT183040.12646808238969953No Hit
CTGCTATCCAAGGCTGTTCAGGTTCCGCCGCGTTGGAAGGACATGGAGTT183020.12645426376181607No Hit
CAGCGGTGCTCTATACCGCTTCGTCACCTGTTGCAACTGCACCCTGCCCA180810.12492730538069043No Hit
CAGCTGGTCAAGAGCGGCATCGTGCTGTCAGGGCATGACATCAGCGACGG179750.12419491810286545No Hit
CAGCATGCAGGCTCGGGCGCGGGCCGTACTTTGGTTGTGGACCAGGCCCA177800.12284760188422518No Hit
CTGCTGCTGTTTGAACAGTAGAATTGTCTCCAGGTTGAGGTGCTTCTCCC176800.1221566704900507No Hit
CAGCGCCATCATCTCCCGTTGTTCCAATAGAATAACTTCCCGGTCTGTGG171810.11870892283311996No Hit
CAGCGTGGTCAGGGTGGGCAGAGGACCATATTTCGCAGTCTGTATGCTCC171750.11866746694946949No Hit
CAGCAACTATGCATCGCAATAGGGAGCCAGTTGGGGAGGAGGGGAACTGG170950.11811472183412991No Hit
CAGCGCTAACATGATGGCTTGTAATTCTGTCTTACTAATGGGATTAATGC170950.11811472183412991No Hit
CTGCACCTGCCGGCGGAGGAGGCCTTCCCCTTCCTGCGCGGCCTCACGCC170840.1180387193807707No Hit
CAGCGACTTTCCGCGCCTGCCTCATGACACTCGCACAGCCCACACCCTTT169580.11716814582411084No Hit
CAGCTGAGGGGCAACCTGAACTTTACCAACTCCGTCTCCCTAAAGCTGAT167250.11555827567568426No Hit
CTGCCTGTGGCAGAGGGATGGGTATTTGCAAAGGGAGGCGAGGAGATGGA166740.11520590066465526No Hit
CTGCCCCACCAGAAGGCATTCCTGACTTCCCTGATATGGCCAGGCATCGA163040.11264945450620964No Hit
CTGCTCTATGGTATCATGCACCTCGCTGACTCCACCCACAAAATCCCCTA162350.11217271184422924No Hit
CAGCACTACCGTGAGGTCAGTATATACTAACACGAATGCCCAGGCGGCTA162300.11213816527452052No Hit
CAGCCTGGAGGTCTGGCAGGTGCCTGACGTCTGAGAAAAAGTCCTGGGTG162150.11203452556539434No Hit
CTGCTTAAACTCTTGAAGTTTGTTTGCAAAATACTGCCACCAGCGATTAG160870.11115013338085099No Hit
CTGCCCCAATATGCCAACCTTTCCATCACTGACCCATTTTATCAACCTGC160350.11079084905588024No Hit
CTGCAAATTGCAAAAAGTAGAGCGGGTCGGATCGGTCAAATCCCATGTCA156040.10781293474698818No Hit
CTGCAGCACTACCGTGAGGTCAGTATATACTAACACGAATGCCCAGGCGG155050.10712891266675542No Hit
CTGCTATCTTTAGAGGGGAAAAGAGGAATAAGCCCCCAGACAGGGGAGTG154690.10688017736485261No Hit
CAGCGGGCCAATTCGTAGAGCCACCAAGTGGCATCAGCCTCAAGGATGGC153120.10579541507599866No Hit
CAGCCGGATGCACCGCCCGGCGTGATACACGCCGCTGTCAAAAAGCGGGG151660.1047866552405039No Hit
CAGCGCTCAAATTGGTGATGAGACAAGTAGCGGATGATAAGGCGAAACAT151280.10452410131071758No Hit
CAGCCACGCCACGCGGCCTCTGGCATCCCCCTGTGGCTGTTTCTTAGCGC150810.10419936355545557No Hit
CAGCAGTGACTCGGGCAACAGTGACACCGAGCAGAGCGAGCGGGAAGAGG146240.10104180708407813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)