FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005322518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14473217
Sequences flagged as poor quality0
Sequence length5-126
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCGGGCGAGTCGCCTTCCCTCTCCCCTGGCCTCTCCTTCCCGCCTC1276560.8820153805473931No Hit
CAGCTTTTAGCCATGCCATGCTCTATAAATCACTTCCCTATCTCAGGTAG1016660.702442311201442No Hit
CTGCAGGGGGGACTGACACCGGGCCGCCGGGGTCCCTCCGGCCGGCCTGA967170.6682481165037462No Hit
CAGCACCAGGCACTGCAGAGTGACCACTAACCTTCGCTCCATACCTAAGG837810.5788692313533336No Hit
CAGCTTCTCTGTCATTCGATCCCAGGTACGATAGGGCAAGGGAGGAGCCC801510.5537884217447994No Hit
CAGCCCGGGGTAGGTCCCCTGGACCTGCCGCTGGCGGACTACGCCTTCGT675790.46692452686918184No Hit
CAGCTCGTTGGAGAGGACCCGGCGAAGGGCGGGCCAGATTTGGAACTCGA672140.464402627280445No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN637280.44031675887952215No Hit
CAGCGGCTTGACGCTCAAGGGCGGCCTGTAACTTAAGTGATGGTCTCCCT625900.4324539596138164No Hit
CAGCTGGCACTAAGGGCTCCTGCTGGTCAGCAGGGGCCGACAAAGCAAAT609180.4209015867032188No Hit
CAGCTTAAAGGTGCCGGCGGAAAAGGTCACGTGACACCTACGGCCACCTG606520.41906370919471464No Hit
CAGCTGGATGTACAGCTACACCGATCACCAGACGACTCCCACATTCGTAC583850.4034002944887788No Hit
CAGCGGTGGCGGCGGCCCCGCGGACAGACGTCGAGGCATACTGCCGGTCC573450.3962146079893641No Hit
CAGCACTTGAATCTGACTCCGATTGCGTAGCTCTAGCAGGGTAAAGAGCC558270.38572626942579524No Hit
CAGCCCACCCACACTTCCCGAGGATGCTACCCTTCTAATCAAATGGTTGG541250.37396661709694534No Hit
CAGCGGCTAGGTTCAGCCAGGCACCCGCGCCCCCAGCTAAGGCCAGGGCT539380.37267457538983906No Hit
CAGCCGCCAAGTGGATCCGGGGCATCTGCGAGGCCGTGCGGGGTCTTTCC517710.35770209207807774No Hit
CAGCGGGGAGTGACCATCACTATGCCGGTGGCCTCCGACTACCTCCCGGA509540.35205718258767216No Hit
CAGCACCTGATCCGTGAAATCACCCCCATCGTGACGCATCGAGAGGCAAA504730.3487338025816928No Hit
CTGCTGTCTGGCTTACGTAAACGCGCTGGACTGAGAAGGTGGCCTAGCAA502050.34688210644530515No Hit
CAGCTCCTGGCAGTCATTCATTCGGATAACGCCGAGTAGAAGCCGACGCA476120.32896625539436053No Hit
CAGCGTGGAGTTGTAGCGCCGCTGATTCTGGAGGATTAGCCGGAGTTCCC468660.32381190719381875No Hit
CAGCTTTGGCAGACCACCTGTGCTGGAAAGAGAGGCTGGAAGATGAGGCC465800.32183584340647975No Hit
CAGCAGTAATAGATGAAAACAAAGCTCAGCATGAAACAGCGTGGAGGCTC452690.3127777328288521No Hit
CAGCACCTCCCGTCCGGTCACGTCTCATGTTGGCATCATCGATCCCGGCT433700.2996569456534784No Hit
CAGCGCTACCTGCGTTTCCAAACGGCCTTTGATTACAACCAGGAGGACCT424560.29334183271072356No Hit
CAGCGAGGCCACCACGCTGGAAAGTGACTCGGGGCGGTGAAAGAGACCAC420660.290647200273443No Hit
CTGCGCTCCTCCACCTCAAACACCAGGCCGGGGTGCTCTGCAAACATCTC413310.2855688545262605No Hit
CAGCGGGGGGCTCATTGCTATGCTCGCCGATGCCGCCGAGAAGGACCTAT411320.2841939010518532No Hit
CAGCACCGTCAAGGTGACGGTGCCCCAGGAGGCCTTTGTGGATCTGGACG375720.2595967434192412No Hit
CAGCCATGGCCCCCTCCTGGATGAAGAGCGAGGTATGCGGTGAGAGAATG372690.2575032212948925No Hit
CAGCGCCTCAGCCATTGCCAGCCTGGTTCCCTCCCTCAGATCCAACGACA366210.2530259858606418No Hit
CAGCCACTCGCGGGTCCCCGTAAAACATATGGAAAGGAATGGCGTGAAAG361250.24959896614553628No Hit
CTGCGCACCAGTAGGTTGGCTAGCGTGCTGGCCCGCGCGGCATCTTCCTC358860.2479476401134592No Hit
CAGCTTTACATAGAGGGTCTCCGTCATGTCATCAATGTTGGTCACCTTCA335770.23199403422197012No Hit
CAGCCCCTTCATCCTTTTGAAGGAGGCCTGCAACGCCCACTTTTGGCGCG328720.22712296789303993No Hit
CTGCAGGGCAGGGTCAGCCCACGGCCGCGGACATCAAGGCCATGGCCGTG325710.2250432643965747No Hit
CAGCTGATCATCCTCTGTGTCAGTGGTGGAGGACAGAGGGAGGCGGCGGC320050.22113259270554708No Hit
CTGCTGCTATCTTTAGAGGGGAAAAGAGGAATAAGCCCCCAGACAGGGGA311260.21505930575075327No Hit
CAGCTCCTTCTTGGGGTCCTTGCGCTGTGAGTTCTGCTCCAGATGGGCCA311250.21505239643681154No Hit
CTGCTTGTCTCCGCAAAACCCGGCGGAGAGAACGTTAAGGTTTCAGGTAC299920.20722414374081452No Hit
CAGCCCTGCGCGCCAGACGGCCGCCGCACTCAGCGAGGAGGCCTGCGCGT297630.20564191084815492No Hit
CAGCTGGGGCCAGGGGAGTCGGCAGGTTACCCACCATTAGCCGGTGCACA296450.204826611803029No Hit
CAGCGCCTCAGACTCCCAAGAGGAAAAAGGGATTGGGCAAAGATAGCCCC294480.20346547695650527No Hit
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT290640.20081230040287518No Hit
CAGCCGTGACTAGCACGAACTGGGCCTCGTGGGAGCCAAAGGTGCTAATG290560.20075702589134123No Hit
CAGCACTCCTCCCTTGAGATGAGTCTGGGCATGGTCATAGCACTTGGACA285670.19737837137382797No Hit
CTGCAGGCCCAGCCCCCCGACGAAGCATACTCCACCGCCACCCCCGGCGC280870.19406190068179036No Hit
CAGCTGGGGCTTCTTAGGCCAGAGCGCCGAGTCCGAGGCCAGCCTGGGCG279870.19337096928761588No Hit
CAGCGCCTCTACCAGAACATTTTCATGTGCTACCGCACGCTGGAGCATGC277300.19159527560458742No Hit
CAGCTGAGACTCCATGGAGCGGATCTTCTTTAGATAGAGCTCCCTCTCCT270500.18689694212420085No Hit
CAGCCAGGCGTTGAAGAACCAGTAGCAAAAGACCACGGCTGTTAGAATGT268770.18570163081227897No Hit
CTGCGTCCCAAGATCGTGGGCTGTCTGCGCCGCATCTACCGCGTTAACAT268340.18540453031278395No Hit
CAGCGTGGTCAGGGTGGGCAGAGGACCATATTTCGCAGTCTGTATGCTCC266610.1842092190008621No Hit
CAGCTTCGGCCTCGGCCATCTGGAGGCGGCCCTGGACGTGCTGGCATCCT265070.18314518465383336No Hit
CAGCTATGGACCACCAATGTGATCGTGGTGGACGAAGCTGGAACGCTTTC259120.17903414285849512No Hit
CAGCATCCCTGATATTCCTTGCGGTATATGGTGTGATGTCGTGTGGAGGC256790.17742427271006853No Hit
CTGCGGAGTTGAGCCCGACGAACTCCTCCAGCAGTGGCGAGTTGAGTGTC248160.17146153477834264No Hit
CAGCAGACATTCATCATTTAGAAATGTATCCAAGATTTCATTAAGTTCGG243640.16833852487667395No Hit
CAGCAGCTGGCCCCGAATCCCGCCGACAAGCGGACAGAGCAGACTGTTTT237950.16440712524382106No Hit
CAGCACCTCAGCTGTTCCCAGTCTCCGACATAACCCAGAATCAACAGACT236190.16319108599007395No Hit
CAGCTGGTCAAGAGCGGCATCGTGCTGTCAGGGCATGACATCAGCGACGG235840.16294926000211288No Hit
CAGCCATATGTGGACAGATGGACCTGAGCCACCCGCCCCCTCGTGGCCAT228570.1579261887664643No Hit
CTGCACCCCCTTTATGATATTTACATTGGCAGGGATGCCGGGGAGAGGGC227720.15733889708141596No Hit
CTGCCCTCTTCGATGATGCGCCGGTACGCGGCCGCCAGGGCCCGGGTCTC224420.15505882348064015No Hit
CAGCATGCAGGCTCGGGCGCGGGCCGTACTTTGGTTGTGGACCAGGCCCA207690.14349954125610084No Hit
CTGCACAGGGCCTAAAGAAAAAGGTCTCCAACACGGTGGTCAAGTGTCTG205930.1422835020023537No Hit
CAGCGCCATCATCTCCCGTTGTTCCAATAGAATAACTTCCCGGTCTGTGG205560.14202785738650917No Hit
CAGCGGGCCAATTCGTAGAGCCACCAAGTGGCATCAGCCTCAAGGATGGC205170.14175839414278113No Hit
CAGCTCCTGTATTTAACTTTGCGGACAGAGGCCAGAGCCGTTTGCTCCAT197910.1367422322210743No Hit
CAGCCCGCACACCGCTCACCAGGTGGCAATTCTACCTCTAAACAGGAGGA197400.1363898572100453No Hit
CAGCCGGATGCACCGCCCGGCGTGATACACGCCGCTGTCAAAAAGCGGGG194690.1345174331318324No Hit
CAGCGGTTGGGCCGAGGCGAGGAGGAGGCGTCCCGGGAGAGCCCGGAGGT194430.13433779096934703No Hit
CAGCTTGGCCAGACCCTCGGTGGCCACCTGGCGCAGGTACTGCTCCTTGC194030.13406141841167724No Hit
CAGCGCTCAAATTGGTGATGAGACAAGTAGCGGATGATAAGGCGAAACAT191200.1321060825661634No Hit
CAGCTGTCTCCCCGGGCTGGCCAAATAGACGGTTGCGGTCCACCAGGTTG184990.12781539860833982No Hit
CTGCCCTGACGGCCATCGTGAGCGAGTACGTGGATCGGACGCTACCCGAA184710.12762193781797093No Hit
CAGCGACTTTCCGCGCCTGCCTCATGACACTCGCACAGCCCACACCCTTT183520.1267997294589033No Hit
CTGCCTTGTCCGGCCATCTTTTAGACACGGAAGACAATGTGCCGCCATGG182640.12619170983202974No Hit
CAGCCGATAGCACCCTAGATCTAGTGAATCATGGCGAGCCCGGAAGAGAG179160.12378726858030249No Hit
CAGCAGTGACTCGGGCAACAGTGACACCGAGCAGAGCGAGCGGGAAGAGG175640.12135519007280829No Hit
CTGCTTGTGATCATGGAAACCACTCAGACTCTCCGCTTTAAGACCAAGGC174120.12030497435366304No Hit
CTGCCCGGCCAGGGCCATCTCCACCAGGCAGGTCACCAGGCCCCCGTCGC171420.11843945958939191No Hit
CAGCAACTATGCATCGCAATAGGGAGCCAGTTGGGGAGGAGGGGAACTGG169980.11744451838178063No Hit
CAGCAACGACGGGGAAGACGCGCGCCGCCTCACCCGCCACCGCATCGCCG168870.11667758453424695No Hit
CAGCGCTAACATGATGGCTTGTAATTCTGTCTTACTAATGGGATTAATGC167120.11546845459444158No Hit
CAGCGGTGCTCTATACCGCTTCGTCACCTGTTGCAACTGCACCCTGCCCA164300.11352002806286951No Hit
CTGCTTCGGGAATACGTCTCTGCGCCCAAAGCCCTGATGGAAGACCTGGC164200.11345093492345205No Hit
CTGCAGCACCTCGGCGCACAGGTTGGCGGCGTTCATGGCCTCGCCCTGCA163900.11324365550519971No Hit
CAGCAGACGGCGGATATGGGAATTTCAGAATGAGGTGGCGGATTCAGGCG162870.11253199616919997No Hit
CAGCACTACCGTGAGGTCAGTATATACTAACACGAATGCCCAGGCGGCTA162220.11208289076298655No Hit
CAGCCACGCCACGCGGCCTCTGGCATCCCCCTGTGGCTGTTTCTTAGCGC161900.11186179271685072No Hit
CAGCCTGGAGGTCTGGCAGGTGCCTGACGTCTGAGAAAAAGTCCTGGGTG160450.1108599421952977No Hit
CAGCTGAGGGGCAACCTGAACTTTACCAACTCCGTCTCCCTAAAGCTGAT158510.10951953529059917No Hit
CTGCTCTATGGTATCATGCACCTCGCTGACTCCACCCACAAAATCCCCTA156200.10792348377005609No Hit
CAGCGCCCGGCACAGAATTTGCTGGCTAGATGCCAGTGAGCCCCCGGGTA155980.10777147886333771No Hit
CAGCTCTAACTTGGCATCTCTTTTACAGTGGTTCTCCTGATTTGCTCTTC155870.10769547640997852No Hit
CAGCCACCTGCGGACAAAAATCAGGCGTTTAGATGGGGCATTTATGTTTG154230.10656234892353234No Hit
CTGCTGCCCCCTCCGGCCCGGCGTCTAAATATTTCCCGGGGGTCCGAATT154200.10654162098170711No Hit
CAGCAGACAAGGACAGGTGCCAGATGTGCCTGGGCTATCGTCCTCCGTCC153550.10609251557549368No Hit
CTGCCACCCGGTTTAATAATTCTGAGTCCGGGCACCGTCCCTGGCCCCCT150050.10367425569588296No Hit
CTGCAGAAGCTCATGGGCCTAACGGCCTGCCTGCGCCGCATGCGTCACAA149470.10327351548726174No Hit
CAGCTCCCACCATCAGCCACGACACTCGAACCAGAAAAAAACCATCCCCC149270.10313532920842684No Hit
CTGCAAATTGCAAAAAGTAGAGCGGGTCGGATCGGTCAAATCCCATGTCA148750.10277604488345611No Hit
CTGCTATCTTTAGAGGGGAAAAGAGGAATAAGCCCCCAGACAGGGGAGTG147630.10200220172198067No Hit
CTGCTCCTACAACAAATCTGGCCCCCGACCCGAAAGGAGATTAACCCTCG145610.10060652030574821No Hit
CTGCACCTGCCGGCGGAGGAGGCCTTCCCCTTCCTGCGCGGCCTCACGCC145440.10048906196873854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)