FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319476
Sequences flagged as poor quality0
Sequence length20-137
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT149130.4492576539188715No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC52270.15746461188452635No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG37650.11342151592600759No Hit
CTGCTGTCTACTTTCTATTTGTAATCCCGTTTCCAACGAAATCCTCAGAA35110.10576970582103923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT60600.097.817473
CATAGTA58700.095.557634
CTGCATA81050.094.96871
ATAGTAT58250.093.865685
CGATACG904.0563464E-1092.59296130-131
TGCATAG67800.090.30062
CTGCTAT70100.089.020621
TGCTATA48800.087.265052
GCTATAA46350.087.04213
TAGTATT64600.085.735876
AGTATTC64200.085.44197
GTATTCC63300.085.2577448
CAGCTGC43000.082.84981
AGCACTA45250.082.510292
CAGCAGC52750.080.283261
CTGCTGC42300.078.782741
CTGCAGC38400.078.028891
CTGCAGT109200.074.214221
TATTCCA74800.072.938459
CAGCACT108400.072.8030851
Produced by FastQC (version 0.11.5)