FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11559241
Sequences flagged as poor quality0
Sequence length20-121
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT379130.32798866292345663No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG159710.13816651110570322No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC152390.13183391539288783No Hit
CTGCATCAAAAGAAAGGTTCAACTCTGTTAGTTGAGGACACACATCACAA148820.1287454773198344No Hit
CAGCTTTGAAACTCTATTTTTGTGGATTCTGCAAATGGATATTTAGATTG118520.10253268359055755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT161450.085.38523
CTGCATA231500.083.570771
CAGCTGC127500.083.4239651
CTGCTGC137150.082.766911
CATAGTA155250.082.1147164
CTGCTAT228400.081.62271
CTGCAGC139150.079.81241
TGCATAG179750.079.5766142
GCTATAA144400.079.5180743
CAGCAGC146650.079.414821
TGCTATA152600.079.214832
ATAGTAT157300.078.477235
CTGCAGT416300.074.120541
GTATTCC173350.070.926998
AGCTGCC39800.070.6173552
TAGTATT180050.069.804056
AGTATTC179500.069.470687
AGCAGCA68400.067.978372
AGCTGCA50600.067.73242
AGCACTA110700.066.948292
Produced by FastQC (version 0.11.5)