FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10857316
Sequences flagged as poor quality0
Sequence length10-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT369960.3407471975578495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCC30300.097.561532
CAGCTGC107850.096.011271
CAGCAGC127850.093.4521941
AGCAGCC33850.092.9640052
CTGCTGC98750.090.430851
CTGCAGC96350.090.005371
TGCTGCC28300.089.5906452
TGCAGCC30000.087.879432
CTGCATA212950.087.399591
GCATAGT151800.087.132613
AGCAGCA55150.086.2503742
GCTGCTA13200.085.839283
CATAGTA144600.083.63524
AGCTGCA35900.083.280522
GCAGCAA31100.081.524223
TGCATAG166150.081.295552
AGCTGCT37250.079.961122
ATAGTAT147050.079.6093445
CTGCTAT188250.079.558561
GCTGCTT20250.078.114693
Produced by FastQC (version 0.11.5)