FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005322533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12774649
Sequences flagged as poor quality0
Sequence length6-121
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAGGGGGGACTGACACCGGGCCGCCGGGGTCCCTCCGGCCGGCCTGA1160530.9084633166829085No Hit
CAGCACCAGGCACTGCAGAGTGACCACTAACCTTCGCTCCATACCTAAGG912450.7142662001907057No Hit
CAGCCCGGGCGAGTCGCCTTCCCTCTCCCCTGGCCTCTCCTTCCCGCCTC912040.7139452520378446No Hit
CAGCTTTTAGCCATGCCATGCTCTATAAATCACTTCCCTATCTCAGGTAG734000.5745754736588066No Hit
CAGCCCGGGGTAGGTCCCCTGGACCTGCCGCTGGCGGACTACGCCTTCGT728310.5701213395373916No Hit
CAGCGGTGGCGGCGGCCCCGCGGACAGACGTCGAGGCATACTGCCGGTCC619790.4851718430776454No Hit
CAGCTTCTCTGTCATTCGATCCCAGGTACGATAGGGCAAGGGAGGAGCCC593660.46471726933554103No Hit
CAGCTGGCACTAAGGGCTCCTGCTGGTCAGCAGGGGCCGACAAAGCAAAT560950.43911186914020106No Hit
CAGCGGCTTGACGCTCAAGGGCGGCCTGTAACTTAAGTGATGGTCTCCCT552100.4321840858406364No Hit
CAGCTGGATGTACAGCTACACCGATCACCAGACGACTCCCACATTCGTAC546190.42755773563719834No Hit
CAGCTTAAAGGTGCCGGCGGAAAAGGTCACGTGACACCTACGGCCACCTG525340.41123634786364777No Hit
CAGCTCGTTGGAGAGGACCCGGCGAAGGGCGGGCCAGATTTGGAACTCGA522970.40938111098003555No Hit
CAGCTCCTGGCAGTCATTCATTCGGATAACGCCGAGTAGAAGCCGACGCA495750.3880732848315441No Hit
CAGCCGCCAAGTGGATCCGGGGCATCTGCGAGGCCGTGCGGGGTCTTTCC495600.3879558647756193No Hit
CAGCGGCTAGGTTCAGCCAGGCACCCGCGCCCCCAGCTAAGGCCAGGGCT485480.38003392500255784No Hit
CAGCACCTGATCCGTGAAATCACCCCCATCGTGACGCATCGAGAGGCAAA481210.37669136741056447No Hit
CTGCGCTCCTCCACCTCAAACACCAGGCCGGGGTGCTCTGCAAACATCTC461450.36122323204340095No Hit
CAGCACTTGAATCTGACTCCGATTGCGTAGCTCTAGCAGGGTAAAGAGCC458050.3585617107757716No Hit
CAGCCCACCCACACTTCCCGAGGATGCTACCCTTCTAATCAAATGGTTGG429620.3363066961761532No Hit
CAGCTTTGGCAGACCACCTGTGCTGGAAAGAGAGGCTGGAAGATGAGGCC424590.33236921030080746No Hit
CTGCTGTCTGGCTTACGTAAACGCGCTGGACTGAGAAGGTGGCCTAGCAA422730.33091320160733967No Hit
CAGCACCTCCCGTCCGGTCACGTCTCATGTTGGCATCATCGATCCCGGCT419410.3283143043695369No Hit
CAGCGTGGAGTTGTAGCGCCGCTGATTCTGGAGGATTAGCCGGAGTTCCC405790.31765256329156283No Hit
CAGCCATGGCCCCCTCCTGGATGAAGAGCGAGGTATGCGGTGAGAGAATG400440.3134645812969108No Hit
CTGCGCACCAGTAGGTTGGCTAGCGTGCTGGCCCGCGCGGCATCTTCCTC393830.3082902708324902No Hit
CTGCAGGGCAGGGTCAGCCCACGGCCGCGGACATCAAGGCCATGGCCGTG391760.3066698740607276No Hit
CAGCGGGGGGCTCATTGCTATGCTCGCCGATGCCGCCGAGAAGGACCTAT382770.29963249870896647No Hit
CAGCGGGGAGTGACCATCACTATGCCGGTGGCCTCCGACTACCTCCCGGA381210.29841133012734833No Hit
CAGCAGTAATAGATGAAAACAAAGCTCAGCATGAAACAGCGTGGAGGCTC340600.26662180698663424No Hit
CAGCCACTCGCGGGTCCCCGTAAAACATATGGAAAGGAATGGCGTGAAAG339770.26597208267718353No Hit
CTGCTTGTCTCCGCAAAACCCGGCGGAGAGAACGTTAAGGTTTCAGGTAC327370.25626535805406475No Hit
CAGCGCTACCTGCGTTTCCAAACGGCCTTTGATTACAACCAGGAGGACCT321580.2517329438953665No Hit
CTGCTGCTATCTTTAGAGGGGAAAAGAGGAATAAGCCCCCAGACAGGGGA306920.24025709042964702No Hit
CTGCAGGCCCAGCCCCCCGACGAAGCATACTCCACCGCCACCCCCGGCGC302280.2366248966997058No Hit
CAGCGCCTCAGACTCCCAAGAGGAAAAAGGGATTGGGCAAAGATAGCCCC300390.23514540399505302No Hit
CAGCGAGGCCACCACGCTGGAAAGTGACTCGGGGCGGTGAAAGAGACCAC299120.2341512475215562No Hit
CTGCGGAGTTGAGCCCGACGAACTCCTCCAGCAGTGGCGAGTTGAGTGTC296320.2319594064776261No Hit
CAGCGCCTCTACCAGAACATTTTCATGTGCTACCGCACGCTGGAGCATGC288970.2262058237373097No Hit
CAGCACCGTCAAGGTGACGGTGCCCCAGGAGGCCTTTGTGGATCTGGACG284490.22269887806702166No Hit
CAGCGCCTCAGCCATTGCCAGCCTGGTTCCCTCCCTCAGATCCAACGACA275150.21538752258476926No Hit
CTGCACCCCCTTTATGATATTTACATTGGCAGGGATGCCGGGGAGAGGGC267120.209101635590927No Hit
CAGCTGATCATCCTCTGTGTCAGTGGTGGAGGACAGAGGGAGGCGGCGGC264350.20693327855818194No Hit
CTGCGTCCCAAGATCGTGGGCTGTCTGCGCCGCATCTACCGCGTTAACAT262340.20535984980878927No Hit
CTGCCCTGACGGCCATCGTGAGCGAGTACGTGGATCGGACGCTACCCGAA251430.1968194977411904No Hit
CAGCTCCTTCTTGGGGTCCTTGCGCTGTGAGTTCTGCTCCAGATGGGCCA250190.1958488252788785No Hit
CTGCCCTCTTCGATGATGCGCCGGTACGCGGCCGCCAGGGCCCGGGTCTC247530.19376657628714494No Hit
CAGCCGTGACTAGCACGAACTGGGCCTCGTGGGAGCCAAAGGTGCTAATG246600.19303857194041105No Hit
CAGCTATGGACCACCAATGTGATCGTGGTGGACGAAGCTGGAACGCTTTC243870.19090152692257925No Hit
CTGCACAGGGCCTAAAGAAAAAGGTCTCCAACACGGTGGTCAAGTGTCTG239080.1871519131367132No Hit
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT238170.18643956479743592No Hit
CTGCAGCACCTCGGCGCACAGGTTGGCGGCGTTCATGGCCTCGCCCTGCA234030.18319877125391076No Hit
CAGCCAGGCGTTGAAGAACCAGTAGCAAAAGACCACGGCTGTTAGAATGT227580.17814970884914333No Hit
CTGCCACCCGGTTTAATAATTCTGAGTCCGGGCACCGTCCCTGGCCCCCT225120.1762240199319762No Hit
CAGCCATATGTGGACAGATGGACCTGAGCCACCCGCCCCCTCGTGGCCAT221840.17365643470908673No Hit
CTGCCTTGTCCGGCCATCTTTTAGACACGGAAGACAATGTGCCGCCATGG220900.1729206023586245No Hit
CAGCTTTACATAGAGGGTCTCCGTCATGTCATCAATGTTGGTCACCTTCA220560.17265445023186154No Hit
CAGCAGCTGGCCCCGAATCCCGCCGACAAGCGGACAGAGCAGACTGTTTT217600.17033736112827835No Hit
CTGCCCGGCCAGGGCCATCTCCACCAGGCAGGTCACCAGGCCCCCGTCGC214460.16787936795758537No Hit
CAGCCCCTTCATCCTTTTGAAGGAGGCCTGCAACGCCCACTTTTGGCGCG211390.16547617081299063No Hit
CAGCTGGGGCCAGGGGAGTCGGCAGGTTACCCACCATTAGCCGGTGCACA211360.16545268680180567No Hit
CAGCAGACATTCATCATTTAGAAATGTATCCAAGATTTCATTAAGTTCGG210900.16509259863030287No Hit
CAGCCCTGCGCGCCAGACGGCCGCCGCACTCAGCGAGGAGGCCTGCGCGT208420.1631512537056791No Hit
CAGCTTCGGCCTCGGCCATCTGGAGGCGGCCCTGGACGTGCTGGCATCCT206270.16146823290408996No Hit
CAGCAACGACGGGGAAGACGCGCGCCGCCTCACCCGCCACCGCATCGCCG199820.15641917049932252No Hit
CTGCTTGTGATCATGGAAACCACTCAGACTCTCCGCTTTAAGACCAAGGC192440.15064210374782117No Hit
CAGCACTCCTCCCTTGAGATGAGTCTGGGCATGGTCATAGCACTTGGACA192360.1505794797179946No Hit
CTGCTCCTACAACAAATCTGGCCCCCGACCCGAAAGGAGATTAACCCTCG191330.14977319533397748No Hit
CTGCTTCGGGAATACGTCTCTGCGCCCAAAGCCCTGATGGAAGACCTGGC187170.14651674578299567No Hit
CTGCTGCCCCCTCCGGCCCGGCGTCTAAATATTTCCCGGGGGTCCGAATT185780.14542865326475898No Hit
CAGCTCCTGTATTTAACTTTGCGGACAGAGGCCAGAGCCGTTTGCTCCAT180580.14135809132603175No Hit
CAGCCCGCACACCGCTCACCAGGTGGCAATTCTACCTCTAAACAGGAGGA179280.14034045084134994No Hit
CTGCAGAAGCTCATGGGCCTAACGGCCTGCCTGCGCCGCATGCGTCACAA177960.13930715434921145No Hit
CAGCTGAGACTCCATGGAGCGGATCTTCTTTAGATAGAGCTCCCTCTCCT176820.1384147619241828No Hit
CAGCTTGGCCAGACCCTCGGTGGCCACCTGGCGCAGGTACTGCTCCTTGC175840.13764761755880728No Hit
CAGCGGTTGGGCCGAGGCGAGGAGGAGGCGTCCCGGGAGAGCCCGGAGGT174870.1368883011971601No Hit
CAGCTGGGGCTTCTTAGGCCAGAGCGCCGAGTCCGAGGCCAGCCTGGGCG172000.1346416641271318No Hit
CTGCTGAACCTGCTGGTCAAGCTCTCGGAGAACAAGACCATCAGCGGGGT171230.134038907840051No Hit
CAGCAGACGGCGGATATGGGAATTTCAGAATGAGGTGGCGGATTCAGGCG167770.13133041855005176No Hit
CAGCATCCCTGATATTCCTTGCGGTATATGGTGTGATGTCGTGTGGAGGC167280.13094684636736398No Hit
CAGCACCTCAGCTGTTCCCAGTCTCCGACATAACCCAGAATCAACAGACT165790.12978047381184407No Hit
CTGCCCCTGGAGAACACATGCGCCCCGGAGCATCTCTCCACTTCCAGAGA160840.12590561196632485No Hit
CAGCATGCAGGCTCGGGCGCGGGCCGTACTTTGGTTGTGGACCAGGCCCA159330.12472358340334831No Hit
CAGCGGTGCTCTATACCGCTTCGTCACCTGTTGCAACTGCACCCTGCCCA159030.12448874329149864No Hit
CAGCTGGTCAAGAGCGGCATCGTGCTGTCAGGGCATGACATCAGCGACGG158920.1244026352504871No Hit
CTGCTATCCAAGGCTGTTCAGGTTCCGCCGCGTTGGAAGGACATGGAGTT158190.12383119097831964No Hit
CTGCCCACCTCCCGGTGCCCAGGGTCATCACATACACCGACTGCCCGGGT157940.1236354908851116No Hit
CAGCGACTTTCCGCGCCTGCCTCATGACACTCGCACAGCCCACACCCTTT151740.11878212857355219No Hit
CTGCTGCTGTTTGAACAGTAGAATTGTCTCCAGGTTGAGGTGCTTCTCCC149050.1166763955706337No Hit
CAGCAACTATGCATCGCAATAGGGAGCCAGTTGGGGAGGAGGGGAACTGG148240.11604232726863963No Hit
CAGCGTGGTCAGGGTGGGCAGAGGACCATATTTCGCAGTCTGTATGCTCC147350.11534563493681901No Hit
CTGCACCTGCCGGCGGAGGAGGCCTTCCCCTTCCTGCGCGGCCTCACGCC147300.1153064949181774No Hit
CAGCGCTAACATGATGGCTTGTAATTCTGTCTTACTAATGGGATTAATGC147120.11516559085106762No Hit
CTGCCTGTGGCAGAGGGATGGGTATTTGCAAAGGGAGGCGAGGAGATGGA146780.11489943872430468No Hit
CAGCGCCATCATCTCCCGTTGTTCCAATAGAATAACTTCCCGGTCTGTGG145920.11422623040366901No Hit
CAGCTGAGGGGCAACCTGAACTTTACCAACTCCGTCTCCCTAAAGCTGAT144840.11338080600101028No Hit
CTGCCCCACCAGAAGGCATTCCTGACTTCCCTGATATGGCCAGGCATCGA143490.11232402549768686No Hit
CTGCTCTATGGTATCATGCACCTCGCTGACTCCACCCACAAAATCCCCTA141760.11096978085268722No Hit
CAGCGGGCCAATTCGTAGAGCCACCAAGTGGCATCAGCCTCAAGGATGGC139750.10939635210329458No Hit
CTGCCCCAATATGCCAACCTTTCCATCACTGACCCATTTTATCAACCTGC138760.10862137973419075No Hit
CAGCCTGGAGGTCTGGCAGGTGCCTGACGTCTGAGAAAAAGTCCTGGGTG138600.1084961316745376No Hit
CTGCAAATTGCAAAAAGTAGAGCGGGTCGGATCGGTCAAATCCCATGTCA138110.10811255949184984No Hit
CAGCACTACCGTGAGGTCAGTATATACTAACACGAATGCCCAGGCGGCTA138000.1080264514508383No Hit
CTGCAGCACTACCGTGAGGTCAGTATATACTAACACGAATGCCCAGGCGG136120.10655478674991382No Hit
CTGCTATCTTTAGAGGGGAAAAGAGGAATAAGCCCCCAGACAGGGGAGTG134650.10540407020185057No Hit
CAGCCGGATGCACCGCCCGGCGTGATACACGCCGCTGTCAAAAAGCGGGG133530.10452733378427854No Hit
CTGCTTAAACTCTTGAAGTTTGTTTGCAAAATACTGCCACCAGCGATTAG132390.10363494135924987No Hit
CAGCCACGCCACGCGGCCTCTGGCATCCCCCTGTGGCTGTTTCTTAGCGC131340.1028130009677761No Hit
CAGCGCTCAAATTGGTGATGAGACAAGTAGCGGATGATAAGGCGAAACAT130440.10210848063222716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)