FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12774649
Sequences flagged as poor quality0
Sequence length6-126
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCGGGCGAGTCGCCTTCCCTCTCCCCTGGCCTCTCCTTCCCGCCTC1153400.9028819500246152No Hit
CAGCTTTTAGCCATGCCATGCTCTATAAATCACTTCCCTATCTCAGGTAG893340.6993068850658832No Hit
CTGCAGGGGGGACTGACACCGGGCCGCCGGGGTCCCTCCGGCCGGCCTGA863270.6757680778548201No Hit
CAGCACCAGGCACTGCAGAGTGACCACTAACCTTCGCTCCATACCTAAGG742740.5814171489173596No Hit
CAGCTTCTCTGTCATTCGATCCCAGGTACGATAGGGCAAGGGAGGAGCCC716460.5608451551193305No Hit
CAGCTCGTTGGAGAGGACCCGGCGAAGGGCGGGCCAGATTTGGAACTCGA606660.47489367418235917No Hit
CAGCCCGGGGTAGGTCCCCTGGACCTGCCGCTGGCGGACTACGCCTTCGT600380.46997768784097316No Hit
CAGCGGCTTGACGCTCAAGGGCGGCCTGTAACTTAAGTGATGGTCTCCCT555100.4345324869591329No Hit
CAGCTGGCACTAAGGGCTCCTGCTGGTCAGCAGGGGCCGACAAAGCAAAT548330.42923292843505917No Hit
CAGCTTAAAGGTGCCGGCGGAAAAGGTCACGTGACACCTACGGCCACCTG540190.42286093340020536No Hit
CAGCTGGATGTACAGCTACACCGATCACCAGACGACTCCCACATTCGTAC521490.4082225664282439No Hit
CAGCGGTGGCGGCGGCCCCGCGGACAGACGTCGAGGCATACTGCCGGTCC518060.4055375611494296No Hit
CAGCGGCTAGGTTCAGCCAGGCACCCGCGCCCCCAGCTAAGGCCAGGGCT494650.38721220442142873No Hit
CAGCCCACCCACACTTCCCGAGGATGCTACCCTTCTAATCAAATGGTTGG486350.3807149613269218No Hit
CAGCACTTGAATCTGACTCCGATTGCGTAGCTCTAGCAGGGTAAAGAGCC483680.3786248843314599No Hit
CAGCCGCCAAGTGGATCCGGGGCATCTGCGAGGCCGTGCGGGGTCTTTCC475160.3719554251549299No Hit
CAGCGGGGAGTGACCATCACTATGCCGGTGGCCTCCGACTACCTCCCGGA462020.3616694282559153No Hit
CAGCACCTGATCCGTGAAATCACCCCCATCGTGACGCATCGAGAGGCAAA452540.35424848072146636No Hit
CTGCTGTCTGGCTTACGTAAACGCGCTGGACTGAGAAGGTGGCCTAGCAA445400.34865928605944474No Hit
CAGCTCCTGGCAGTCATTCATTCGGATAACGCCGAGTAGAAGCCGACGCA426400.33378607897563367No Hit
CAGCGTGGAGTTGTAGCGCCGCTGATTCTGGAGGATTAGCCGGAGTTCCC423510.33152378589814874No Hit
CAGCTTTGGCAGACCACCTGTGCTGGAAAGAGAGGCTGGAAGATGAGGCC416690.3261850873554334No Hit
CAGCAGTAATAGATGAAAACAAAGCTCAGCATGAAACAGCGTGGAGGCTC402380.3149832140202052No Hit
CAGCACCTCCCGTCCGGTCACGTCTCATGTTGGCATCATCGATCCCGGCT392040.30688905816512063No Hit
CAGCGCTACCTGCGTTTCCAAACGGCCTTTGATTACAACCAGGAGGACCT383730.30038398706688535No Hit
CAGCGAGGCCACCACGCTGGAAAGTGACTCGGGGCGGTGAAAGAGACCAC383160.29993779085437106No Hit
CAGCGGGGGGCTCATTGCTATGCTCGCCGATGCCGCCGAGAAGGACCTAT374980.29353448380460395No Hit
CTGCGCTCCTCCACCTCAAACACCAGGCCGGGGTGCTCTGCAAACATCTC368440.2884149693662816No Hit
CAGCACCGTCAAGGTGACGGTGCCCCAGGAGGCCTTTGTGGATCTGGACG335130.2623398889472423No Hit
CAGCCATGGCCCCCTCCTGGATGAAGAGCGAGGTATGCGGTGAGAGAATG330920.2590442993776189No Hit
CAGCGCCTCAGCCATTGCCAGCCTGGTTCCCTCCCTCAGATCCAACGACA327970.256735038277764No Hit
CAGCCACTCGCGGGTCCCCGTAAAACATATGGAAAGGAATGGCGTGAAAG326360.25547472967750423No Hit
CTGCGCACCAGTAGGTTGGCTAGCGTGCTGGCCCGCGCGGCATCTTCCTC320090.2505665713398466No Hit
CAGCCCCTTCATCCTTTTGAAGGAGGCCTGCAACGCCCACTTTTGGCGCG298820.2339164074097065No Hit
CTGCAGGGCAGGGTCAGCCCACGGCCGCGGACATCAAGGCCATGGCCGTG295270.23113746608615238No Hit
CAGCTGATCATCCTCTGTGTCAGTGGTGGAGGACAGAGGGAGGCGGCGGC291430.22813151265447684No Hit
CAGCTTTACATAGAGGGTCTCCGTCATGTCATCAATGTTGGTCACCTTCA289620.22671464397965063No Hit
CAGCTCCTTCTTGGGGTCCTTGCGCTGTGAGTTCTGCTCCAGATGGGCCA278470.21798641982257203No Hit
CTGCTGCTATCTTTAGAGGGGAAAAGAGGAATAAGCCCCCAGACAGGGGA273950.21444816213737067No Hit
CAGCCCTGCGCGCCAGACGGCCGCCGCACTCAGCGAGGAGGCCTGCGCGT273320.2139549979024864No Hit
CTGCTTGTCTCCGCAAAACCCGGCGGAGAGAACGTTAAGGTTTCAGGTAC268550.210221040124077No Hit
CAGCTGGGGCCAGGGGAGTCGGCAGGTTACCCACCATTAGCCGGTGCACA267510.20940692773633154No Hit
CAGCGCCTCAGACTCCCAAGAGGAAAAAGGGATTGGGCAAAGATAGCCCC266470.20859281534858612No Hit
CAGCCGTGACTAGCACGAACTGGGCCTCGTGGGAGCCAAAGGTGCTAATG262110.20517980572303784No Hit
CTGCAGGCCCAGCCCCCCGACGAAGCATACTCCACCGCCACCCCCGGCGC250600.1961697734317397No Hit
CAGCGCCTCTACCAGAACATTTTCATGTGCTACCGCACGCTGGAGCATGC248830.19478421677182675No Hit
CAGCACTCCTCCCTTGAGATGAGTCTGGGCATGGTCATAGCACTTGGACA248620.194619828693532No Hit
CAGCTGGGGCTTCTTAGGCCAGAGCGCCGAGTCCGAGGCCAGCCTGGGCG248320.19438498858168235No Hit
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT244590.1914651431910184No Hit
CAGCTTCGGCCTCGGCCATCTGGAGGCGGCCCTGGACGTGCTGGCATCCT241850.18932027016945827No Hit
CAGCGTGGTCAGGGTGGGCAGAGGACCATATTTCGCAGTCTGTATGCTCC240510.1882713176698632No Hit
CTGCGTCCCAAGATCGTGGGCTGTCTGCGCCGCATCTACCGCGTTAACAT238820.18694838503977682No Hit
CAGCCAGGCGTTGAAGAACCAGTAGCAAAAGACCACGGCTGTTAGAATGT238770.18690924502113523No Hit
CAGCTGAGACTCCATGGAGCGGATCTTCTTTAGATAGAGCTCCCTCTCCT233990.1831674592389975No Hit
CAGCTATGGACCACCAATGTGATCGTGGTGGACGAAGCTGGAACGCTTTC228350.17875246513622411No Hit
CAGCATCCCTGATATTCCTTGCGGTATATGGTGTGATGTCGTGTGGAGGC225260.17633361198417272No Hit
CAGCAGACATTCATCATTTAGAAATGTATCCAAGATTTCATTAAGTTCGG217940.1706035132550413No Hit
CTGCGGAGTTGAGCCCGACGAACTCCTCCAGCAGTGGCGAGTTGAGTGTC215660.16881872840498396No Hit
CAGCAGCTGGCCCCGAATCCCGCCGACAAGCGGACAGAGCAGACTGTTTT214650.16802810002842347No Hit
CAGCTGGTCAAGAGCGGCATCGTGCTGTCAGGGCATGACATCAGCGACGG213900.16744099974879936No Hit
CAGCACCTCAGCTGTTCCCAGTCTCCGACATAACCCAGAATCAACAGACT206470.1616247929786564No Hit
CTGCACCCCCTTTATGATATTTACATTGGCAGGGATGCCGGGGAGAGGGC205760.16106900471394556No Hit
CAGCCATATGTGGACAGATGGACCTGAGCCACCCGCCCCCTCGTGGCCAT205510.1608733046207375No Hit
CTGCCCTCTTCGATGATGCGCCGGTACGCGGCCGCCAGGGCCCGGGTCTC202640.1586266675507092No Hit
CAGCATGCAGGCTCGGGCGCGGGCCGTACTTTGGTTGTGGACCAGGCCCA191570.1499610674234572No Hit
CAGCGGGCCAATTCGTAGAGCCACCAAGTGGCATCAGCCTCAAGGATGGC190390.14903736298351525No Hit
CAGCGCCATCATCTCCCGTTGTTCCAATAGAATAACTTCCCGGTCTGTGG184430.14437187276143557No Hit
CAGCTCCTGTATTTAACTTTGCGGACAGAGGCCAGAGCCGTTTGCTCCAT178620.13982380259528068No Hit
CAGCCCGCACACCGCTCACCAGGTGGCAATTCTACCTCTAAACAGGAGGA177090.1386261180248475No Hit
CTGCACAGGGCCTAAAGAAAAAGGTCTCCAACACGGTGGTCAAGTGTCTG176860.13844607393909608No Hit
CAGCTTGGCCAGACCCTCGGTGGCCACCTGGCGCAGGTACTGCTCCTTGC173780.13603504879077305No Hit
CAGCCGGATGCACCGCCCGGCGTGATACACGCCGCTGTCAAAAAGCGGGG173670.1359489407497615No Hit
CAGCGGTTGGGCCGAGGCGAGGAGGAGGCGTCCCGGGAGAGCCCGGAGGT173010.13543229250369226No Hit
CAGCGCTCAAATTGGTGATGAGACAAGTAGCGGATGATAAGGCGAAACAT169350.13256724313912654No Hit
CTGCCCTGACGGCCATCGTGAGCGAGTACGTGGATCGGACGCTACCCGAA167740.13130693453886677No Hit
CAGCGACTTTCCGCGCCTGCCTCATGACACTCGCACAGCCCACACCCTTT167390.13103295440837553No Hit
CAGCTGTCTCCCCGGGCTGGCCAAATAGACGGTTGCGGTCCACCAGGTTG167090.13079811429652588No Hit
CTGCCTTGTCCGGCCATCTTTTAGACACGGAAGACAATGTGCCGCCATGG162690.12735379265606436No Hit
CAGCCGATAGCACCCTAGATCTAGTGAATCATGGCGAGCCCGGAAGAGAG159320.12471575539961999No Hit
CAGCAACGACGGGGAAGACGCGCGCCGCCTCACCCGCCACCGCATCGCCG157150.1230170785905742No Hit
CAGCAGTGACTCGGGCAACAGTGACACCGAGCAGAGCGAGCGGGAAGAGG157070.12295445456074762No Hit
CTGCCCGGCCAGGGCCATCTCCACCAGGCAGGTCACCAGGCCCCCGTCGC155440.12167848995303121No Hit
CAGCAACTATGCATCGCAATAGGGAGCCAGTTGGGGAGGAGGGGAACTGG154610.1210287656435805No Hit
CTGCTTGTGATCATGGAAACCACTCAGACTCTCCGCTTTAAGACCAAGGC151100.11828113633493961No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN150640.11792104816343682No Hit
CAGCAGACGGCGGATATGGGAATTTCAGAATGAGGTGGCGGATTCAGGCG149250.1168329556452001No Hit
CTGCAGCACCTCGGCGCACAGGTTGGCGGCGTTCATGGCCTCGCCCTGCA148710.11641024344387074No Hit
CAGCGCTAACATGATGGCTTGTAATTCTGTCTTACTAATGGGATTAATGC147460.11543174297783054No Hit
CAGCGGTGCTCTATACCGCTTCGTCACCTGTTGCAACTGCACCCTGCCCA145570.11395225027317776No Hit
CAGCACTACCGTGAGGTCAGTATATACTAACACGAATGCCCAGGCGGCTA143730.11251189758716658No Hit
CAGCCTGGAGGTCTGGCAGGTGCCTGACGTCTGAGAAAAAGTCCTGGGTG142610.11163516116959457No Hit
CAGCCACGCCACGCGGCCTCTGGCATCCCCCTGTGGCTGTTTCTTAGCGC142600.11162733316586625No Hit
CTGCTTCGGGAATACGTCTCTGCGCCCAAAGCCCTGATGGAAGACCTGGC142320.11140814906147323No Hit
CTGCTCTATGGTATCATGCACCTCGCTGACTCCACCCACAAAATCCCCTA141450.11072711273710925No Hit
CAGCGCCCGGCACAGAATTTGCTGGCTAGATGCCAGTGAGCCCCCGGGTA141180.11051575663644456No Hit
CTGCTGCCCCCTCCGGCCCGGCGTCTAAATATTTCCCGGGGGTCCGAATT139910.10952160016294774No Hit
CAGCTGAGGGGCAACCTGAACTTTACCAACTCCGTCTCCCTAAAGCTGAT139630.10930241605855472No Hit
CAGCAGACAAGGACAGGTGCCAGATGTGCCTGGGCTATCGTCCTCCGTCC137290.10747066318612745No Hit
CAGCTCCCACCATCAGCCACGACACTCGAACCAGAAAAAAACCATCCCCC136030.10648433471635893No Hit
CTGCAGAAGCTCATGGGCCTAACGGCCTGCCTGCGCCGCATGCGTCACAA135310.1059207184479198No Hit
CTGCTCCTACAACAAATCTGGCCCCCGACCCGAAAGGAGATTAACCCTCG135010.10568587833607013No Hit
CAGCCACCTGCGGACAAAAATCAGGCGTTTAGATGGGGCATTTATGTTTG134940.10563108230997188No Hit
CTGCAAATTGCAAAAAGTAGAGCGGGTCGGATCGGTCAAATCCCATGTCA134470.10526316613474077No Hit
CTGCTATCTTTAGAGGGGAAAAGAGGAATAAGCCCCCAGACAGGGGAGTG133270.10432380568734217No Hit
CTGCCACCCGGTTTAATAATTCTGAGTCCGGGCACCGTCCCTGGCCCCCT131920.10326702518401874No Hit
CAGCTCTAACTTGGCATCTCTTTTACAGTGGTTCTCCTGATTTGCTCTTC131560.10298521704979917No Hit
CTGCACCTGCCGGCGGAGGAGGCCTTCCCCTTCCTGCGCGGCCTCACGCC130360.10204585660240058No Hit
CAGCAGCCACCTGCGGACAAAAATCAGGCGTTTAGATGGGGCATTTATGT128350.10047242785300795No Hit
CAGCTCGCCTTCCCCCTCCAGTAATCCCCATCCCTCACCAATCACCACCC127990.10019061971878836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGAGT70800.0198.90642120
GGGACCG103700.0168.46541120
TCCGAAT91450.0156.03363120
CTTAGTG91850.0155.93489120
GACGGTC28600.0152.00914120
CTACCCC161600.0121.80445120
GCGTCAA121050.0119.31137120
GTCCTTC111450.0106.49472120
TATCCGA58750.0105.46242118-119
CCTTGAT103250.0104.490456120
AAGCGAC15700.0102.77888120
GGTGTCA208550.0102.24838120
ATCCGAA61900.0100.47118118-119
GCGAGTC149150.0100.1373759
CCGGTGT135750.097.66386118-119
GATCCGT59800.097.633279
CGGTGTC135850.096.95019118-119
CAGCGGT132800.096.8322751
TGATCCG64050.091.415428
GCGCGCA43150.091.17156120
Produced by FastQC (version 0.11.5)