FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9581423
Sequences flagged as poor quality0
Sequence length6-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT485190.5063861599680965No Hit
CTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTTGGATTAGCGTTT183640.19166255367287302No Hit
CAGCCATTCAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATC132160.13793358251691842No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA127540.13311175177215326No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA99660.1040137774942198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT202450.092.023543
GCTGCTA34350.091.1825943
CTGCATA267250.089.359961
CATAGTA199700.088.352164
TGCATAG220650.087.576632
CTGCTAT243400.085.648391
CTGCTGC129050.085.452351
TGCTATA174150.084.916262
GCTATAA166700.084.64793
ATAGTAT203950.084.199475
CAGCTGC93000.083.0910341
CAGCAGC108500.078.313011
GTATTCC219250.077.92838
CTGCAGC105800.076.931091
AGTATTC230850.075.749527
TAGTATT231450.075.248796
AGCACTA122250.073.8476942
CTGCAGT340300.073.362461
TGCTGCG10050.071.7127462
CTGCGGT63200.071.5099641
Produced by FastQC (version 0.11.5)