FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11535166
Sequences flagged as poor quality0
Sequence length12-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT405650.3516637731958083No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC211990.183777155872746No Hit
CAGCTTTGAAACTCTATTTTTGTGGATTCTGCAAATGGATATTTAGATTG168150.14577163432238424No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG118060.10234789859114295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC142600.088.381361
GCATAGT156400.086.960483
CAGCAGC163000.085.158741
CTGCATA215700.084.383321
CATAGTA152750.081.553294
TGCATAG171350.080.910422
AGCTGCC40800.080.075062
ATAGTAT152150.079.479855
CTGCTAT197150.079.219291
GCTATAA127450.078.660993
CTGCTGC114400.078.229331
TGCTATA133550.077.375932
CTGCAGC118200.074.576511
AGCACTA131200.074.490612
TAGTATT171300.072.1161656
CTGCAGT374300.072.028821
GTATTCC169800.071.843358
AGTATTC172450.071.687157
AGCAGCA77550.071.611172
AGCTGCT50300.071.524682
Produced by FastQC (version 0.11.5)