FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4065736
Sequences flagged as poor quality0
Sequence length10-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT70350.093.287885
GTATTCC74300.088.1483158
TAGTATT76150.087.007226
AGTATTC77050.084.921127
TATTCCA90700.072.5567559
CATAGTA38900.072.3997344
ACGATCA8800.071.94095145
TATAAAG65400.057.0932585
CTTTGAA57200.056.568943145
AATAGTA41050.053.6528324
ATAACCG4800.052.7769931
TAAAGAC71200.052.703877
CGTACTA2800.052.010872
ACGCAGC3000.050.665911
CGCACTA4400.050.366262
TATAGTA22800.050.242394
ACTATTC68550.049.299715
CGCGATA1053.6798156E-948.253251
AAGACAC78300.047.6806349
CTATTCA74250.046.1083226
Produced by FastQC (version 0.11.5)