FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9420221
Sequences flagged as poor quality0
Sequence length8-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT454560.482536450047191No Hit
CAGCCATTCAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATC167970.17830791867834098No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA157910.1676287636988559No Hit
CTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTTGGATTAGCGTTT144830.15374373913308403No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCATTATTCACAA111570.11843671183510451No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA108290.1149548402314553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAT21600.0152.2093120
GCATAGT186750.091.8924263
GCTATAA175050.090.922863
CATAGTA180900.089.144724
CTGCATA246250.088.712581
CTGCTAT239550.088.3936541
TGCTATA184500.088.1451952
TGCATAG201500.086.8301852
AGCACTA182250.086.769432
ATAGTAT181450.086.243065
GTATTCC197650.079.548188
ACACCAT272850.078.382965120
CAGCACT368950.077.806321
AGACTTA96650.077.76899120
AGTATTC207300.077.600057
TAGTATT208000.077.174936
CTGCTGC122100.076.770791
GCTGCTA34900.076.225033
CAGCTGC114650.075.859221
CAGCAGC132850.075.17981
Produced by FastQC (version 0.11.5)