FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4134578
Sequences flagged as poor quality0
Sequence length10-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT75750.092.466215
GTATTCC78400.090.2263268
AGTATTC81500.087.881287
TAGTATT82000.087.50086
TATTCCA96950.073.745069
CATAGTA38700.073.086244
GATAGTA21800.064.146784
CGGACTA5600.063.3112452
ACGATCA9250.058.64322145
CTTTGAA59500.057.475494145
TATAAAG64450.056.6462255
CGAACTA3650.055.505752
AAGCTCG16050.054.006729
TAAAGAC69450.052.475657
AATAGTA41250.051.6945844
CGCACTA6150.051.4725572
ACTATTC66250.051.382415
ATAACCG5050.050.1766741
ATAACTC26500.048.5269971
CGTACTA2350.048.493722
Produced by FastQC (version 0.11.5)