FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975569
Sequences flagged as poor quality0
Sequence length10-137
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT163000.5477943882329732No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA48300.1623218954089117No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA40800.13711663214665834No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCATTATTCACAA30630.10293829516304276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG2900.0138.02127130-131
GCATAGT69250.0101.27433
CTGCATA93200.099.0950851
CATAGTA66300.098.8337254
CTGCTAT78100.095.814731
TGCATAG76200.095.776112
ATAGTAT67900.093.9174355
TGCTATA55550.093.078772
CTGCTGC38050.092.27671
GCTATAA54150.091.23153
GTATTCC71400.089.653248
CTGCAGC34050.087.088321
CAGCTGC26450.086.663121
AGTATTC75150.086.389467
AGCTGCG2950.086.300432
GCTGCTA6550.086.065153
TAGTATT75850.085.6721046
CAGCAGC33800.083.426571
CTGCAGT116950.082.4451451
TATTCCA85100.077.4996269
Produced by FastQC (version 0.11.5)