FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113618
Sequences flagged as poor quality0
Sequence length9-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT41800.091.90215
GGATTGC16000.090.23593145
AGTATTC45750.087.293227
TAGTATT45900.087.144916
GTATTCC45400.086.617048
CATAGTA21850.077.8617554
TATTCCA52900.074.7977459
CGCGATA603.254172E-971.1811451
CGCTATA2150.068.106871
GAGCGAT3850.065.77713140-144
CGCAGCG752.6011548E-1065.061422
GATAGTA11250.060.164724
CGCACTA2750.057.6680682
CGATAGT3100.057.0531543
ACGCAGC2700.056.4929731
CGAACGG1101.03116745E-556.32606130-134
CGCGTAT551.1299917E-555.4658281
TATAGTA11900.054.828744
CGTACGA452.8057612E-454.2178462
CGGTATA2050.053.5718651
Produced by FastQC (version 0.11.5)