FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3374354
Sequences flagged as poor quality0
Sequence length9-137
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT226200.67035053227966No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA43700.12950626994085387No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA42060.1246460804053161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT82850.0102.682683
CTGCATA109050.0100.939911
CATAGTA79250.0100.703994
ATAGTAT78050.099.570975
TGCATAG90050.097.4608152
CTGCTAT82750.094.8498461
CTGCTGC36550.093.458651
GTATTCC83500.093.289758
TGCTATA60350.092.4885562
CAGCTGC29700.091.8500061
GCTATAA58500.091.733233
TAGTATT86100.091.720696
AGTATTC85900.091.5860067
CTGCAGC32750.084.392041
CAGCAGC37850.084.2426761
TGCTGCC12850.083.8017352
TATTCCA94950.082.667629
AGCACTA45000.081.096292
CTGCAGT117150.077.875051
GCTGCTA4250.077.4219063
Produced by FastQC (version 0.11.5)