FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132981
Sequences flagged as poor quality0
Sequence length17-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT40400.094.165755
GTATTCC44350.086.736668
GGATTGC14600.086.577255145
TAGTATT45100.086.514776
AGTATTC45150.086.013247
CATAGTA20650.077.1839754
TATTCCA52600.073.825569
CCGGACT6800.066.519231
GATAGTA13850.064.814814
CGGACTA4000.064.151962
TCGATAG2450.062.3439832
CGATAGT2900.061.0799063
CCGGTTT5050.059.3101731
CGTACTA1600.057.278532
CGGGATA3950.055.7095831
TATAGTA11350.054.879614
GCATAGT12700.054.8276373
TATTCCG2850.053.5587659
CCGGATT4500.052.9756851
CCGGTTA2550.052.7359771
Produced by FastQC (version 0.11.5)