FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16549807
Sequences flagged as poor quality0
Sequence length6-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT747940.45193276272043537No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN546440.3301790770128014No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC242720.14666032057050576No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG173710.10496194910309227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCC42600.098.316962
CAGCTGC168500.095.707371
AGCAGCC46550.095.0918662
CAGCAGC188250.094.4066241
GCATAGT282000.091.3355563
CTGCATA361050.089.362861
CTGCTGC132600.088.404681
GCAGCAA41300.088.26843
TGCTGCC37350.088.255682
CATAGTA271950.087.88914
CTGCAGC131050.085.3920441
ATAGTAT272250.084.966875
TGCATAG305200.084.71682
GCTGCTA17350.082.385293
AGCAGCA87150.082.1318052
TGCAGCC39200.081.970612
CTGCTAT310400.081.7958451
GCTGCAA27200.081.066913
GCTATAA204100.080.321433
TGCTATA212050.080.129382
Produced by FastQC (version 0.11.5)