FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13528459
Sequences flagged as poor quality0
Sequence length9-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT735890.5439570020502705No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA156000.11531246833065023No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA153020.11310970451253909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT295850.092.492713
CTGCATA379900.090.334141
CATAGTA284400.089.8364264
TGCATAG313700.087.143462
ATAGTAT288050.085.869095
CAGCAGC151500.082.368031
CTGCTAT296350.082.181511
CAGCTGC131350.081.851381
CTGCTGC132000.081.4483261
GCTATAA202150.080.702813
TGCTATA210700.080.444942
AGCACTA194600.078.8650052
TAGTATT325550.078.6639566
GTATTCC322200.078.570498
AGTATTC332050.077.646767
CTGCAGC127550.075.217791
CTGCGAT52450.072.711311
CTGCGTA38450.072.623491
CAGCACT442400.071.76381
GCTGCTA20500.071.02123
Produced by FastQC (version 0.11.5)