FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13528459
Sequences flagged as poor quality0
Sequence length9-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT754510.5577205800010185No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA161820.11961451041837064No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA142280.1051708845774674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT287800.093.778893
CATAGTA275150.092.035324
CTGCATA373150.091.9064561
TGCATAG307250.089.238692
CAGCTGC135950.088.527031
ATAGTAT281850.087.1982045
CAGCAGC159500.085.761471
CTGCTAT287200.083.539331
AGCACTA214500.082.8619462
CTGCTGC123650.082.5980451
GCTATAA203600.082.5363543
TGCTATA210450.081.279532
GTATTCC306700.080.632748
AGTATTC314550.079.882397
TAGTATT313100.079.761586
CTGCAGC127050.077.098441
ACACCAT320450.076.04829120
AGCTGCC42500.074.2026142
CAGCACT476350.074.065531
CTGCAGT431300.071.880161
Produced by FastQC (version 0.11.5)