FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13477787
Sequences flagged as poor quality0
Sequence length14-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT755770.560752295610548No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA162500.12056875509310246No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA142310.1055885509987656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT284500.094.3013463
CTGCATA371150.091.940581
CATAGTA277300.091.0622254
TGCATAG303800.089.411192
CAGCTGC131200.089.245171
ATAGTAT279200.088.206275
CAGCAGC157500.085.913341
GCTATAA200750.083.616793
CTGCTGC123100.083.075031
AGCACTA217200.082.466992
TGCTATA207400.082.091192
CTGCTAT288000.082.0088961
GTATTCC303150.081.0875558
TAGTATT314450.079.4401866
AGTATTC318250.078.981787
ACACCAT320450.077.391235120
CTGCAGC116250.075.457821
CAGCACT480550.074.30341
AGCTGCC43700.072.05452
AGCAGCC56800.071.527542
Produced by FastQC (version 0.11.5)