FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14886622
Sequences flagged as poor quality0
Sequence length16-121
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT494670.3322916374178104No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG229140.1539234354173835No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC228730.15364802035008346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC149150.092.7642441
AGCTGCC41650.091.849822
CAGCAGC169450.091.496751
CTGCTGC144050.090.087911
CTGCAGC140550.088.812811
AGCAGCC45250.087.664742
GCATAGT210850.085.30543
CTGCATA293250.084.336251
AGCAGCA74000.084.300642
TGCTGCC43800.083.7433552
TGCAGCC45950.083.4910352
GCAGCAA39950.082.156573
AGCTGCA51900.081.562552
GCTGCTA18350.081.14033
CTGCTAT289050.081.1059341
CATAGTA206800.080.380574
TGCATAG233200.079.24992
GCTGCTT26450.078.685593
GCTATAA183200.078.3073
ATAGTAT207250.077.426755
Produced by FastQC (version 0.11.5)