FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12642807
Sequences flagged as poor quality0
Sequence length9-121
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT453690.3588522707022262No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA177050.14004010343589046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT194500.085.697113
CAGCTGC130350.084.794091
CTGCATA277800.084.0435261
CATAGTA189550.081.99164
CAGCAGC153150.080.669541
CTGCTGC140000.080.6291351
ATAGTAT188800.079.160185
TGCATAG218750.079.0518652
GCTATAA156250.078.921873
CTGCTAT252050.078.9163361
GCTGCTA28300.077.680223
TGCTATA168000.077.2804642
CTGCAGC128250.075.436371
CTGCAGT430850.071.426791
GTATTCC213000.070.962288
TAGTATT214600.070.8663946
CTGCGTG103350.070.433281
AGTATTC218050.069.895627
AGCAGCA83950.067.5548252
CTGCGGT76200.066.748011
Produced by FastQC (version 0.11.5)