FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12642807
Sequences flagged as poor quality0
Sequence length9-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT445130.35208162238021984No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN377280.2984147428652514No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA158820.12562083720806622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC135150.088.1909941
CCGATAT14350.086.92297120
GCATAGT179500.086.6766743
CAGCAGC150250.084.007431
CATAGTA172050.083.5378954
CTGCATA253750.083.3915561
ATAGTAT173000.080.868695
CTGCTAT238450.079.896411
CTGCTGC129750.079.8530651
TGCATAG202300.078.658012
GCTATAA155500.078.314473
TGCTATA160050.077.878932
CTGCAGC116450.072.985121
CTGCAGT409200.072.02861
AGCACTA147250.071.966682
GTATTCC196000.071.50328
TAGTATT198700.071.129346
GCTGCTA25250.071.063043
AGTATTC199150.071.0541847
AGCTGCC48750.070.381932
Produced by FastQC (version 0.11.5)