FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8186342
Sequences flagged as poor quality0
Sequence length6-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT421250.5145765959936685No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA127070.15522195383481413No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA93820.11460552222225752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT185900.092.093783
CTGCATA233100.090.666921
CATAGTA177300.090.557794
TGCATAG196000.088.523762
ATAGTAT182350.085.2537465
CTGCTAT178750.083.9055561
CAGCTGC92600.083.796311
GCTATAA125800.082.9535453
AGCACTA124200.081.196932
TGCTATA131250.081.138952
CAGCAGC110000.078.816771
GTATTCC199950.078.243198
AGTATTC208500.075.586967
TAGTATT208100.075.468746
CTGCTGC82200.073.1836851
CTGCGGT49250.072.70871
CAGCACT288350.072.6478651
CTGCAGC87950.072.57661
CTGCAGT280100.071.596421
AGCTGCC30450.071.135962
Produced by FastQC (version 0.11.5)