FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11281632
Sequences flagged as poor quality0
Sequence length16-121
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT618030.5478196771530928No Hit
CTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTTGGATTAGCGTTT294680.261203343629716No Hit
CAGCCATTCAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATC210680.18674603107068197No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA159350.1412472947176437No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA113810.10088079455171026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA46850.097.358653
GCATAGT252400.092.71853
CTGCTGC156600.091.2204741
CTGCATA336950.090.3213041
CATAGTA247400.089.140174
TGCATAG274100.087.809052
ATAGTAT249850.085.533425
TGCTATA195950.085.425062
CTGCTAT279950.085.335961
GCTATAA187100.084.688433
CAGCTGC100250.084.189321
CAGCAGC110500.081.968681
GTATTCC272650.079.373878
AGTATTC279350.078.807837
CTGCAGC121150.078.519051
TAGTATT282600.077.767336
TGCAGCG11250.077.525642
CTGCGTA26750.075.131851
CTGCGGT70650.074.75951
TGCTGCC44600.074.698572
Produced by FastQC (version 0.11.5)