FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11281632
Sequences flagged as poor quality0
Sequence length16-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT661130.5860233696685019No Hit
CAGCCATTCAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATC376360.3336042161275957No Hit
CTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTTGGATTAGCGTTT179090.15874476316901667No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA134360.11909624423133106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAT28750.0189.83572120
GCTGCTA36050.095.238083
GCATAGT223750.093.405653
CAGCTGC116250.090.798531
CATAGTA213500.090.71774
CTGCATA295050.090.686931
CTGCTGC119200.089.063281
TGCATAG239400.088.851862
CAGCAGC137950.086.567591
ATAGTAT219700.085.986495
AATCCAA126550.084.05128120
AGCACTA190250.083.9642
ATATCGC9700.083.508766120
ACCGATA29000.083.28054118-119
CTGCTAT236100.083.188431
GCTATAA161900.082.701953
TGCTATA168600.082.145972
TAGTATT235200.081.935736
GTATTCC237500.080.171728
AGTATTC241950.079.9407357
Produced by FastQC (version 0.11.5)