FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15735746
Sequences flagged as poor quality0
Sequence length8-121
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT623230.39606002791351613No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG206800.13142052496271864No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC190240.1208967150334023No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA164370.10445643949768889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCC39850.098.4799962
GCTGCTA25900.096.9559563
CAGCTGC152400.095.6235351
CTGCTGC154400.094.42011
CAGCAGC160550.094.358191
TGCAGCC47200.094.315062
TGCTGCC40650.094.135032
AGCAGCC41000.094.126882
CTGCAGC138500.091.837291
GCTGCTT22550.090.389293
GCAGCAA35050.089.788933
GCATAGT255950.087.96043
GCAGCTA12400.087.6678853
GCTGCAA25650.087.51753
CTGCATA342450.085.031031
CATAGTA245950.084.6637654
CTGCTAT325750.084.017161
AGCAGCA73700.083.634362
GCTATAA212900.082.641713
AGCTGCA55200.082.4186552
Produced by FastQC (version 0.11.5)