FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14199609
Sequences flagged as poor quality0
Sequence length6-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT775060.5458319310059876No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA221310.15585640421507382No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA177060.12469357430898273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT333850.092.970333
CTGCATA420500.091.638491
CATAGTA320400.091.3384
CAGCTGC151850.090.433521
TGCATAG354900.089.498482
CAGCAGC170250.087.722761
ATAGTAT333400.085.2897955
CTGCTGC131350.085.1095351
CTGCTAT333200.083.893571
TGCTATA242100.083.617392
GCTATAA235000.083.526633
AGCACTA224250.081.850672
CTGCAGC132500.081.80161
AGCTGCG15450.080.31532
AGCAGCG17400.079.518762
GTATTCC360750.079.478078
GCAGCAA42250.077.452663
TAGTATT374150.077.10126
AGTATTC377100.077.0076457
AGCTGCC46250.075.9777762
Produced by FastQC (version 0.11.5)