FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11191743
Sequences flagged as poor quality0
Sequence length11-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT624630.5581168188011465No Hit
CTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTTGGATTAGCGTTT298600.2668038392232559No Hit
CAGCCATTCAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATC208910.1866644007104166No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA159940.14290892848415124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA45050.096.341273
GCATAGT252250.092.53783
CTGCTGC154100.091.0385741
CTGCATA335300.090.257991
CATAGTA249500.088.258764
TGCATAG271800.088.0746462
CTGCTAT282650.086.940931
ATAGTAT250600.085.838885
GCTATAA187550.085.4585953
CAGCTGC99700.084.2971041
TGCTATA202150.084.245232
CAGCAGC106850.081.293351
CTGCAGC116200.081.187411
GTATTCC277550.078.382478
TAGTATT282100.077.848136
AGTATTC284900.077.083047
CTGCAGT397250.075.309521
TGCAGCG11650.074.416412
TGCTGCT97150.074.3467562
AGCACTA135500.073.3789442
Produced by FastQC (version 0.11.5)