FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3841403
Sequences flagged as poor quality0
Sequence length17-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT80550.098.400075
GTATTCC87100.093.666848
AGTATTC89300.091.565597
TAGTATT89600.090.0956956
ACGATCA6700.081.294334145
TATTCCA101950.080.082229
CATAGTA38450.078.7750244
GAGCGAT5850.074.193565140-144
AATAGTA41250.064.9895864
GTCTACT27000.063.551116
CGGACTA6750.063.3521732
GATAGTA21100.061.6453554
CGTACTA3200.059.180552
CGCGATA1800.057.7280731
CGCACTA6000.057.0169562
TATAAAG63150.055.7941555
TGTCTAC30650.054.987615
TAAAGAC65950.054.353377
TATAGTA21000.053.7967034
CGGTATA3000.052.973991
Produced by FastQC (version 0.11.5)