FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3846492
Sequences flagged as poor quality0
Sequence length20-137
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT186690.4853513279112501No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC60160.15640224911425787No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA52350.13609803426082778No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG43820.11392198397916856No Hit
CTGCATCAAAAGAAAGGTTCAACTCTGTTAGTTGAGGACACACATCACAA39790.1034449051239415No Hit
CTGCTGTCTACTTTCTATTTGTAATCCCGTTTCCAACGAAATCCTCAGAA38680.10055915883875488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT74150.099.3589253
CTGCATA100800.097.512081
CATAGTA72200.096.126934
GCTATAA61200.095.5700763
CTGCTAT91000.094.193931
TGCATAG81850.094.142752
TGCTATA65250.093.54732
ATAGTAT73450.091.2605445
CTGCTGC44950.090.397681
GTATTCC76400.087.34748
AGTATTC77850.087.320627
CAGCTGC45350.086.4610751
TAGTATT80450.084.867236
CAGCAGC54050.084.287261
AGCACTA49900.083.094652
CTGCAGC40550.076.654361
CTATAAA64850.076.653244
GCTGCAA9750.075.4420553
TATTCCA91100.074.816629
ATCGGAA102150.074.47065130-131
Produced by FastQC (version 0.11.5)