FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13064852
Sequences flagged as poor quality0
Sequence length6-121
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT457950.35052061822055086No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA183950.14079761485243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTGC161500.085.698671
GCATAGT197450.084.710093
CTGCATA279000.084.363021
CATAGTA188300.082.55064
CAGCTGC122850.082.035591
CTGCTAT284100.081.775361
GCTATAA173400.081.230363
TGCTATA185550.080.22482
CAGCAGC135800.080.026131
CTGCAGC150950.079.82141
ATAGTAT189500.079.671885
GCTGCTA28800.079.401353
TGCATAG222050.079.126012
CTGCAGT501250.075.6876141
CTGCGGT90950.074.5909961
TGCAGCG12400.073.766422
AGCAGCG13300.072.049682
CTGCGAT47700.070.426781
GTATTCC217450.069.080168
TGCTGCG13700.068.753782
Produced by FastQC (version 0.11.5)