FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13064852
Sequences flagged as poor quality0
Sequence length6-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT499330.3821933842036634No Hit
CTGCGTGCTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACA140150.10727255081037276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAT12550.093.458786120
CAGCTGC141700.088.863221
GCATAGT179250.086.9916153
CAGCAGC163150.085.504361
CTGCATA249800.085.4005051
CATAGTA171700.083.004624
CTGCTGC125700.081.588981
TGCATAG199450.079.6100542
CTGCTAT233850.079.4666751
ATAGTAT174500.079.270615
AGCAGCG15650.077.138312
CTGCAGC117250.076.410211
TGCTATA161500.075.857542
GCTATAA158000.075.840313
AGCTGCG12850.074.809382
AGCACTA159200.074.776872
AGCTGCC48200.073.74642
GTATTCC190650.073.586668
CTGCAGT435400.072.7950741
GCTGCTA23200.072.7530753
Produced by FastQC (version 0.11.5)