FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4380991
Sequences flagged as poor quality0
Sequence length13-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT90350.099.455415
GTATTCC93800.095.656378
AGTATTC96300.094.375337
TAGTATT100000.091.336
CTTTGAA68200.081.43469145
CGTACTA3550.077.6626742
TATTCCA117650.076.971339
CATAGTA44350.075.871594
CGATAGT6400.072.1182863
CGCACTA6600.070.917122
TATAAAG86050.069.12035
GATAGTA25950.068.6627044
CGCGATA2450.068.068821
TAAAGAC88450.068.041887
AAGCTCG21550.066.616159
ACGATCA10550.065.309105145
CGGACTA6500.062.144032
AAGACAC95150.061.4951369
CCGTACT6700.061.269751
AATAGTA50900.060.5676574
Produced by FastQC (version 0.11.5)