FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3117608
Sequences flagged as poor quality0
Sequence length18-137
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT172200.5523465426057413No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA38310.12288267158667798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT68650.0100.364153
CTGCATA91900.098.8796841
CATAGTA66450.098.642264
TGCATAG72750.098.165372
CTGCTGC31750.095.024951
ATAGTAT67650.093.970955
CAGCTGC30400.093.534291
CTGCTAT74750.092.976221
TGCTATA53200.089.905452
GCTATAA51100.089.732913
CAGCAGC33150.089.386621
CTGCAGC29650.088.026331
GTATTCC73050.085.711968
CGATACG1052.5465852E-1184.34471130-131
TAGTATT76200.083.741196
AGTATTC76300.083.474537
CTGCAGT107700.078.37081
AGCACTA41600.078.137112
GCAGCAA8450.077.9255453
GCTGCTT6300.077.91433
Produced by FastQC (version 0.11.5)