FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3471560
Sequences flagged as poor quality0
Sequence length20-137
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT160530.4624145917109311No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC51800.14921245779995163No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA37510.10804940718293793No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG36870.10620585558077636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT59600.098.600673
CATAGTA56950.096.95734
CTGCATA80100.095.895061
ATAGTAT57000.094.023065
TGCTATA53350.093.466932
GCTATAA52050.093.142063
TGCATAG65600.092.792852
CTGCTAT77350.091.761291
GTATTCC61900.086.581458
TAGTATT64600.085.290526
CAGCTGC46200.084.2364651
AGTATTC64950.083.256547
AGCACTA43400.081.216772
CTGCTGC41850.078.331931
CAGCAGC52050.077.658031
CTGCAGC41150.074.402191
CGAACGG2400.073.77009130-131
CTGCAGT118600.072.271651
CTATAAA55400.071.766754
TATTCCA76300.071.2669149
Produced by FastQC (version 0.11.5)