FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3471560
Sequences flagged as poor quality0
Sequence length20-151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT67500.097.1880655
TAGTATT72750.093.034536
GTATTCC72450.092.198418
AGTATTC73700.091.0643167
TATTCCA87300.077.453119
CATAGTA32900.075.8068854
ACGATCA6400.068.35399145
TATAAAG52400.066.874865
GATAGTA20350.066.708554
CGCACTA5200.064.3732152
TAAAGAC56650.061.743647
AATAGTA34050.061.0079234
CGCGATA1600.059.2385371
GTCTACT19650.057.513916
CGTACTA2100.057.1435172
AAGACAC61450.057.123739
CGGACTA5700.055.40232
ATAACCG4200.054.160951
CGATAGT6350.053.7042773
CGGGATA5900.053.548961
Produced by FastQC (version 0.11.5)