FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23162678
Sequences flagged as poor quality0
Sequence length5-121
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT896990.38725660305772935No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC289280.12489056748964866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC266250.089.493991
GCATAGT377900.089.400033
CAGCAGC323200.086.822821
CTGCATA498050.086.168821
CATAGTA363100.085.894654
TGCATAG412750.082.944272
AGCTGCC77600.082.893172
ATAGTAT364400.082.72175
GCTGCTA37900.082.5402453
CTGCTGC238250.082.173851
AGCAGCC91050.080.9735952
CTGCAGC220250.080.901711
CTGCTAT429350.079.417261
GCTATAA281500.078.903393
TGCTATA290700.078.430252
AGCACTA305000.077.889522
GTATTCC404350.075.82788
GCAGCAA68950.075.590423
AGTATTC414950.074.380977
TAGTATT413450.074.225326
Produced by FastQC (version 0.11.5)