FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16810700
Sequences flagged as poor quality0
Sequence length28-121
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT700130.41647879029427687No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG209560.12465869951875888No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC193250.11495654553349949No Hit
CTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAA171320.10191128269495023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCC43050.095.2355652
AGCAGCC45900.092.980092
CAGCTGC161100.092.755641
CAGCAGC179900.090.34811
TGCTGCC44150.090.286642
CTGCTGC162450.089.984421
GCATAGT292350.089.071293
CTGCAGC153550.089.02741
TGCAGCC47350.088.988942
CTGCATA384750.087.5861741
CATAGTA281200.086.4398354
GCTGCTA17250.084.7717363
GCAGCAA41350.084.088373
ATAGTAT282850.083.810175
TGCATAG317000.083.7342
CTGCTAT344650.083.20931
GCTGCAA27600.082.416963
AGCAGCA79900.082.0191042
GCTGCTT26500.081.9548953
GCTATAA225800.081.791263
Produced by FastQC (version 0.11.5)