FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005322656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005322656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19044839
Sequences flagged as poor quality0
Sequence length8-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCT824690.4330254511471586No Hit
CAGCTAACAGAGTTGAACCTTTCTATTGACAGAGCAGTTTTGAAACAGTC219220.11510730019823219No Hit
CTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATCCTCCAAG212170.11140550991268552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCC49850.0104.202052
CCGATAT17300.0103.74972120
AGCAGCC51800.0103.308322
CAGCTGC175750.0101.390141
GCTGCTA26250.0100.968943
TGCAGCC45300.0100.690352
TGCTGCC44450.098.707842
CAGCAGC200250.097.9398651
CTGCTGC170150.097.84411
CTGCAGC140450.094.197251
GCAGCAA36200.093.184553
GCATAGT326850.092.490883
GCTGCAA27100.091.1853943
CTGCATA429300.090.4035641
GCAGCAT23950.090.076433
CATAGTA313550.089.210224
AGCTGCA60550.088.8420942
GCAGCTA13850.088.603383
GCTGCTT26950.088.3661963
GCTGCAC26700.088.144263
Produced by FastQC (version 0.11.5)