EGAF00005323607 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005323607
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2836
Sequences flagged as poor quality	0
Sequence length	150-151
%GC	35

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	2212	77.99717912552892	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT	113	3.984485190409027	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT	83	2.926657263751763	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT	51	1.7983074753173485	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGTGGT	42	1.4809590973201692	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT	14	0.4936530324400564	No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTTGGAGTTTTGTGTGGT	11	0.38787023977433005	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTTGGAGTTTTGTGTGGT	11	0.38787023977433005	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT	10	0.3526093088857546	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGCGTGGT	10	0.3526093088857546	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTTGGAGTTTTGTGTGGT	8	0.2820874471086037	No Hit
GAAAATTTATAAGATTGTGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	8	0.2820874471086037	No Hit
GAAAATTTATAAGATTGCGTAAGGTTGTAAGGTTTGGAGTTTTGTGTGGT	7	0.2468265162200282	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTTGGAGTTTTGTGTGGT	7	0.2468265162200282	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGCAAGGTTTGGAGTTTTGTGTGGT	7	0.2468265162200282	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTCTTGTGTGGT	6	0.21156558533145275	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGGGTTTTGTGTGGT	6	0.21156558533145275	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGCTTTGTGTGGT	6	0.21156558533145275	No Hit
GAAAATTTATAAGATTGCGTAAAGCTGTAAGGTTTGGAGTTTTGTGTGGT	6	0.21156558533145275	No Hit
GAAAATTTATAAGATTACGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	6	0.21156558533145275	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTCTGTGTGGT	6	0.21156558533145275	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT	5	0.1763046544428773	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTACGGTTTGGAGTTTTGTGTGGT	5	0.1763046544428773	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTTGGAGTTTTGTGTGGT	5	0.1763046544428773	No Hit
AAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	5	0.1763046544428773	No Hit
GAACATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	5	0.1763046544428773	No Hit
GAAAATTTATAAGACTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	4	0.14104372355430184	No Hit
GCAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	4	0.14104372355430184	No Hit
GAAAATTTATAAGATTGCGTAAAGTGTAAGGTTTGGAGTTTTGTGTGGTT	4	0.14104372355430184	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGATTGGAGTTTTGTGTGGT	4	0.14104372355430184	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCTGGAGTTTTGTGTGGT	4	0.14104372355430184	No Hit
GAAACTTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	4	0.14104372355430184	No Hit
GAAGATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	4	0.14104372355430184	No Hit
GAAAATTTATAGGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	4	0.14104372355430184	No Hit
GAAAATTTATAAGATTGCATAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	3	0.10578279266572638	No Hit
GAAAATTTATAAGATTGCGTCAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	3	0.10578279266572638	No Hit
GAAAATTTATAAGATTGCGTAGAGTTGTAAGGTTTGGAGTTTTGTGTGGT	3	0.10578279266572638	No Hit
GAAAATTTATAAGATTGCGTGAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	3	0.10578279266572638	No Hit
GAAAATTTATAAGTTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGTT	3	0.10578279266572638	No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGCGTTTTGTGTGGT	3	0.10578279266572638	No Hit
GAGAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	3	0.10578279266572638	No Hit
GAAAATTTATAAGGTTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	3	0.10578279266572638	No Hit
GAAAATTTATAAGATTGCGTTAAGTTGTAAGGTTTGGAGCTTTGTGTGGT	3	0.10578279266572638	No Hit
GAAAATTTATAAGATTGCGTACAGTTGTAAGGTTTGGAGTTTTGTGTGGT	3	0.10578279266572638	No Hit
GAAAATTTATACGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT	3	0.10578279266572638	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAAATT	270	0.0	144.94644	1
TTATAAG	270	0.0	144.94644	7
AAATTTA	275	0.0	144.94643	3
ATAAGAT	265	0.0	144.94643	9
AATTTAT	280	0.0	144.94643	4
TATAAGA	265	0.0	144.94643	8
AAAATTT	275	0.0	144.94643	2
TTTATAA	275	0.0	144.94643	6
ATTTATA	275	0.0	144.94643	5
GTCTCTT	260	0.0	144.3153	145
TTGTTTT	255	0.0	28.989286	70-74
TTGTTTC	280	0.0	28.989286	125-129
GTATGTT	255	0.0	28.989286	95-99
GGTTGTT	280	0.0	28.989286	125-129
GGTTGTA	255	0.0	28.989286	50-54
TGGTTAG	255	0.0	28.989286	45-49
GTTTGGT	265	0.0	28.989286	120-124
GTTTGAT	265	0.0	28.989286	80-84
GGGTTAG	270	0.0	28.989286	135-139
TTCGTTT	25	4.912608E-4	28.989286	70-74

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content