FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2836
Sequences flagged as poor quality0
Sequence length150-151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA236783.46262341325811No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACGACA371.304654442877292No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACATACAACA170.5994358251057829No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACG160.5641748942172073No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACATACAACA150.5289139633286318No Hit
CTAACCCCGAAACAACCAAACCCCACACACCCTCACTAAAACATACAACA140.4936530324400564No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACAACA120.4231311706629055No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAGCA120.4231311706629055No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTGAAACACACAACA110.38787023977433005No Hit
CTAACCCCGAAACAACCACACTCCACACACCCTCACTAAAACATACAACA110.38787023977433005No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAACACATACAACA90.31734837799717913No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAGACATACAACA90.31734837799717913No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCCCACTAAAACATACAACA70.2468265162200282No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAGAACATACAACA70.2468265162200282No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACACACAACA70.2468265162200282No Hit
CTAACCCCGAAACAACCAAACTCCACGCACCCTCACTAAAACATACAACA70.2468265162200282No Hit
CTAACCCCGAAACAACCAACTCCACACACCCTCACTAAAACATACAACAT70.2468265162200282No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATGCAACA70.2468265162200282No Hit
CTAACCCCGAAACAATCAAACTCCACACACCCTCACTAAAACATACAACA60.21156558533145275No Hit
CTAACCCCGAAGCAACCAAACTCCACACACCCTCACTAAAACATACAACA60.21156558533145275No Hit
CTAACCCCGACACAACCAAACTCCACACACCCTCACTAAAACATACAACA60.21156558533145275No Hit
CTAGCCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA50.1763046544428773No Hit
CTAACCCCGAAACAACAAACTCCACACACCCTCACTAAAACATACAACAT50.1763046544428773No Hit
CTAACCCCGAAACAACCAGACTCCACACACCCTCACTAAAACATACAACA50.1763046544428773No Hit
CTAACCCCGAAACAACCAAATTCCACACACCCTCACTAAAACATACAACA50.1763046544428773No Hit
CTAACCCCGAAATAACCAAACTCCACACACCCTCACTAAAACATACAACA50.1763046544428773No Hit
CTAACCCCGAAACACCAAACTCCACACACCCTCACTAAAACATACAACAT40.14104372355430184No Hit
CTAACCTCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA40.14104372355430184No Hit
CTAACCCCGAAACAGCCAAACTCCACACACCCTCACTAAAACATACAACA40.14104372355430184No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCACTAAAACGTACAACG40.14104372355430184No Hit
CTAACCCCGAACAACCAAACTCCACACACCCTCACTAAAACATACAACAT40.14104372355430184No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACCAAAACATACAACA40.14104372355430184No Hit
CCAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA40.14104372355430184No Hit
CTAACCCCGAAACAACTAAACTCCACACACCCTCACTAAAACATACAACA40.14104372355430184No Hit
CTAACCCCGAAACAACCGAACTCCACACACCCTCACTAAAACATACAACA40.14104372355430184No Hit
CTAACCCCGAAACAACCAAGCTCCACACACCCTCACTAAAACATACAACA40.14104372355430184No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACATACAACA30.10578279266572638No Hit
CTAACCCTGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA30.10578279266572638No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG30.10578279266572638No Hit
CTAACTCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA30.10578279266572638No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAGCATACAACA30.10578279266572638No Hit
CTCACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACAACA30.10578279266572638No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACATACGACG30.10578279266572638No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTGAAACATACAACA30.10578279266572638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACAA2700.0144.946449
AACCCCG2700.0144.946443
TAACCCC2700.0144.946442
CCCCGAA2700.0144.946445
CTAACCC2700.0144.946441
CCCGAAA2700.0144.946446
CGAAACA2700.0144.946448
CCGAAAC2700.0144.946447
ACCCCGA2650.0144.946434
GTCTCTT2600.0144.3153145
CTACAAC2550.028.98928680-84
AAACCTT2550.028.989286105-109
ACAAAAC2600.028.98928695-99
ACAAAAA2700.028.98928665-69
AATCTTA2700.028.989286125-129
AAACCAA2600.028.98928670-74
AACAACC2750.028.98928610-14
AACAAAA2650.028.98928665-69
CACACCC2600.028.98928625-29
ATCTTAT2700.028.989286125-129
Produced by FastQC (version 0.11.5)