FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34802
Sequences flagged as poor quality0
Sequence length148-151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT2757679.23682546980059No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT13303.8216194471582097No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT6711.928050112062525No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT4001.149359232228033No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGTGGT2010.5775530141945865No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTTGGAGTTTTGTGTGGT1830.525831848744325No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT1820.5229584506637549No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCTGGAGTTTTGTGTGGT1660.47698408137463366No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGCTTTGTGTGGT1620.4654904890523533No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTTGGAGTTTTGTGTGGT1460.41951611976323194No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGC1430.41089592552152177No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT1050.30170679845985865No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTTGGAGTTTTGTGTGGT1030.2959600022987185No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTCTGTGTGGT880.2528590310901672No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTTGGAGTTTTGTGTGGT870.24998563300959714No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTCTTGTGTGGT860.2471122349290271No Hit
GAAAATTTATAAGATTGCGTACAGTTGTAAGGTTTGGAGTTTTGTGTGGT670.1925176713981955No Hit
GAAAATTTATAAGATTGCGTAAAATTGTAAGGTTTGGAGTTTTGTGTGGT660.18964427331762543No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGCGTGGT660.18964427331762543No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTTGGAGTTTTGTGTGGT640.18389747715648525No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGATTGGAGTTTTGTGTGGT610.175277282914775No Hit
GAAAATTTATAAGATTGCGTAAAGTTGCAAGGTTTGGAGTTTTGTGTGGT590.16953048675363486No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGGGTTTTGTGTGGT580.16665708867306478No Hit
GAAAATTTATAAGATTGCGTAACGTTGTAAGGTTTGGAGTTTTGTGTGGT540.15516349635078444No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT520.14941670018964426No Hit
GCAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT490.14079650594793403No Hit
GAAAATTTATAAGATTGTGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT490.14079650594793403No Hit
GAAAATTTATAAGATTACGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT480.13792310786736395No Hit
GAAAATTTATAAGATTGCATAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT480.13792310786736395No Hit
GAAAATTTATAAGATTGCGTAAAGCTGTAAGGTTTGGAGTTTTGTGTGGT470.13504970978679387No Hit
GAAAATTTATAAGATTGCGCAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT440.1264295155450836No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGCGTTTTGTGTGGT400.11493592322280327No Hit
GAAAATTTATAGGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT390.11206252514223321No Hit
GAAAATTTATACGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT380.10918912706166313No Hit
GAAAATTTATAAGATTGCGTAAAGTCGTAAGGTTTGGAGTTTTGTGTGGT380.10918912706166313No Hit
GAAACTTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT350.10056893281995287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTT303.929017E-10155.19078145
GCCTCTT201.4971265E-6155.19077145
GTCCCTT100.0057620504155.19077145
GCCCCTT201.4971265E-6155.19077145
GCAAATT151.2221248E-4144.933911
AAAATTT34250.0144.933912
CAAATTT151.2221248E-4144.933912
AAATTTC100.0070713223144.93393
TATACGA100.0070713223144.93398
ATAAGAC100.0070713223144.93399
AATTTCT100.0070713223144.93394
TTTCTAA100.0070713223144.93396
GAAAATT34350.0144.93391
TTATCAG100.0070713223144.93397
CTAAGAT100.0070713223144.93399
TTATACG100.0070713223144.93397
ATCAGAT100.0070713223144.93399
TCTAAGA100.0070713223144.93398
ATACGAT100.0070713223144.93399
TATCAGA100.0070713223144.93398
Produced by FastQC (version 0.11.5)