FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28668
Sequences flagged as poor quality0
Sequence length148-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG1959968.36542486395982No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACA7752.7033626342960795No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG6932.4173294265383003No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACG6502.267336402958002No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG6102.1278080089298173No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG5601.9533975163945863No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG5331.8592158504255618No Hit
CTCACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG3051.0639040044649086No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAGAACGTACGACG1670.5825310450676713No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTGAAACGTACGACG1640.5720664155155575No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTGCGACG1490.5197432677549881No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGGCG1270.44300265103948655No Hit
CTAACCCCGAAACAACCAACTCCGCACGCCCTCGCTAAAACGTACGACGT1110.3871912934282126No Hit
CTACCCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG1110.3871912934282126No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCGTACGACG1030.3592856146225757No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACA850.29649783730989254No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCCAAAACGTACGACG830.2895214176084833No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACA790.2755685782056648No Hit
CTAACCCCGAAACAACCAAACCCCGCACGCCCTCGCTAAAACGTACGACG790.2755685782056648No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCCCGCTAAAACGTACGACG730.25463931910143717No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG720.25115110925073253No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACGTACGACG710.2476628994000279No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGCACGACG600.2092925910422771No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCACTAAAACGTACGACG590.20580438119157252No Hit
CTAACCCCGAAACAACAAACTCCGCACGCCCTCGCTAAAACGTACGACGT580.20231617134086788No Hit
CTAACCCCGAAACAACCAAACTCCACACACCCTCGCTAAAACGTACGACG570.19882796149016327No Hit
CTAACCCCGAAACAACCAAACTCTGCACGCCCTCGCTAAAACGTACGACG530.18487512208734477No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACATACGACG530.18487512208734477No Hit
CTAACCCCGAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACGT530.18487512208734477No Hit
CTAACCCCGAAACAACCAAATCCGCACGCCCTCGCTAAAACGTACGACGT490.1709222826845263No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACA430.1499930235802986No Hit
CTAACCCCGACACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG410.14301660387888937No Hit
CTAACCCCGAAACAACCAAACTCCGCGCGCCCTCGCTAAAACGTACGACG410.14301660387888937No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACA400.13952839402818473No Hit
CTAACCCTGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG340.11859913492395702No Hit
CTAACCCCGAAACACCAAACTCCGCACGCCCTCGCTAAAACGTACGACGT330.11511092507325241No Hit
CTAGCCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG330.11511092507325241No Hit
TTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG330.11511092507325241No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACG310.10813450537184316No Hit
CTAACCCCGAAACAATCAAACTCCGCACGCCCTCGCTAAAACGTACGACG310.10813450537184316No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACA310.10813450537184316No Hit
CTAACCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACGT300.10464629552113855No Hit
CTAACCCCGAAACAACTAAACTCCGCACGCCCTCGCTAAAACGTACGACG300.10464629552113855No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACAACG290.10115808567043394No Hit
TAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACGT290.10115808567043394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCTT151.012769E-4151.84459145
CTAACCC27550.0144.954641
ATCCCGA100.0070618135144.954624
AATCCCG100.0070618135144.954623
GAAACAC100.0070618135144.954629
AACCCTG100.0070618135144.954623
CCTGAAA100.0070618135144.954626
AACCCGA151.2191877E-4144.954623
ACCCGAA151.2191877E-4144.954624
TAACCCT100.0070618135144.954622
TAACCCG151.2191877E-4144.954622
TAACCCC27350.0144.954622
CACCCCG550.0144.954623
CTAATCC100.0070618135144.954621
CGAACAA151.2191877E-4144.954628
ACCCTGA100.0070618135144.954624
ACCCCCG202.0983716E-6144.954623
TAATCCC100.0070618135144.954622
CCCTGAA100.0070618135144.954625
GAACAAC151.2191877E-4144.954629
Produced by FastQC (version 0.11.5)