FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27874
Sequences flagged as poor quality0
Sequence length149-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT2086074.83676544450026No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGCGGT18466.622659108846954No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTCGTGCGGT6212.227882614622946No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGTGGT5782.0736169907440627No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGATTCGGAGTTTCGTGCGGT1600.5740116237353807No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTTGTGTGGT1280.45920929898830454No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGC1280.45920929898830454No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGCTCGGAGTTTCGTGCGGT1270.4556217263399584No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGCTTCGTGCGGT1110.39822056396642036No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTCTCGTGCGGT1110.39822056396642036No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGCGGT1000.3587572648346129No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTCCGGAGTTTCGTGCGGT920.33005668364784385No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAAGTTCGGAGTTTCGTGCGGT900.32288153835115163No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGGGTTTCGTGCGGT870.3121188204061132No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCCGTGCGGT860.3085312477577671No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAGGGTTCGGAGTTTCGTGCGGT850.304943675109421No Hit
GAAAATTTATAAGATTGCGTAAAGTTATAAGGTTCGGAGTTTCGTGCGGT720.25830523068092126No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTTGGAGTTTTGTGTGGT600.21525435890076774No Hit
GAAAATTTATAAGATTGCGTAAAGCTGTAAGGTTCGGAGTTTCGTGCGGT510.1829662050656526No Hit
GAAAATTTATAAGATTGCGTAAAATTGTAAGGTTCGGAGTTTCGTGCGGT450.16144076917557582No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGA450.16144076917557582No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGCGCGGT440.15785319652722968No Hit
GAAAATTTATAAGATTGCATAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT420.15067805123053743No Hit
GAAAATTTATAAGATTGTGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT420.15067805123053743No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGG370.13274018798880677No Hit
GAAAATTTATAAGATTGCGTAAAGTGTAAGGTTCGGAGTTTCGTGCGGTT360.12915261534046063No Hit
GAAAATTTATAAGATTGCGTAAAGTCGTAAGGTTCGGAGTTTCGTGCGGT330.11838989739542227No Hit
GAAAATTTATAAGATTGCGTAAGTTGTAAGGTTCGGAGTTTCGTGCGGTT330.11838989739542227No Hit
GAAAATTTATAAGATTACGTAAAGTTGTAAGGTTCGGAGTTTCGTGCGGT310.11121475209873No Hit
GAAAATTTATAAGATTGCGTAAGGTTGTAAGGTTCGGAGTTTCGTGCGGT310.11121475209873No Hit
GAAAATTTATAAGATTGCGTAAAGTTGTAAGGTTCGGAGTTCTGTGCGGT300.10762717945038387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCTT100.006362124150.0855145
ATCTCTT100.006362124150.0855145
GTCTCCT100.006362124150.0855145
TCTCTTT100.006362124150.0855145
GAAAATT27600.0144.96591
AAAATTT27650.0144.965882
AAAAATT100.00705912144.965881
TTTATAA27800.0144.705176
GTCTCTT26500.0144.70508145
AATTTAT27750.0144.704684
ATTTATA27750.0144.704685
AAATTTA27700.0144.704223
ATAAGAT27700.0144.442559
TATAAGA27700.0144.442558
TTATAAG27900.0143.666927
GGCTCTT255.35007E-6120.068405145
TTCTCTT203.3346697E-4112.564125145
TGTTGTA255.138438E-428.9931890-94
CGTTTGA26050.028.9931880-84
TTGTTTC26650.028.99318125-129
Produced by FastQC (version 0.11.5)