FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005323614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005323614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27874
Sequences flagged as poor quality0
Sequence length147-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG1934869.4123556002009No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACG8062.8915835545669797No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACA6652.385735811150176No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCGCTAAAACGTACGACG5742.059266700150678No Hit
CTAACCCCGAAACAACCAAACTCCACACGCCCTCGCTAAAACGTACGACG5381.9301140848102174No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACG4811.7256224438544883No Hit
CTCACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG3931.4099160508000288No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACGACG3931.4099160508000288No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTGAAACGTACGACG1770.6350003587572648No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAGCGTACGACG1670.5991246322738035No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGGCG1410.5058477434168043No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAGACGTACGACG1320.4735595895816891No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTGCGACG1270.4556217263399584No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCCAAAACGTACGACG940.3372318289445361No Hit
CTAACCCCGAAACAACCAACTCCGCACGCCCTCGCTAAAACGTACGACGT880.31570639305445936No Hit
CTAACCCCGAAACAACCAAACCCCGCACGCCCTCGCTAAAACGTACGACG810.29059338451603645No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAGAACGTACGACG720.25830523068092126No Hit
CTAGCCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG690.2475425127358829No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGCACGACG620.22242950419746No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACGTACGACA600.21525435890076774No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACAACA590.2116667862524216No Hit
CTAACCCCGAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACGT570.20449164095572933No Hit
CTAACCCCGACACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG550.1973164956590371No Hit
CTAACCCCGAAACAACAAACTCCGCACGCCCTCGCTAAAACGTACGACGT520.18655377771399873No Hit
CTAACCCCGAAACAACCAAACTCTGCACGCCCTCGCTAAAACGTACGACG510.1829662050656526No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCCCGCTAAAACGTACGACG510.1829662050656526No Hit
CTACCCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG450.16144076917557582No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACAACA420.15067805123053743No Hit
CTGACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG400.14350290593384518No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACGTACGACA400.14350290593384518No Hit
CTAACCCCGAAACAACCAAATCCGCACGCCCTCGCTAAAACGTACGACGT380.1363277606371529No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCACTAAAACATACGACG360.12915261534046063No Hit
CTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACATACAACA360.12915261534046063No Hit
CTAACTCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG350.12556504269211452No Hit
TTAACCCCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG340.12197747004376838No Hit
CTAACCCCGAAACAATCAAACTCCGCACGCCCTCGCTAAAACGTACGACG310.11121475209873No Hit
CTAACCTCGAAACAACCAAACTCCGCACGCCCTCGCTAAAACGTACGACG310.11121475209873No Hit
CTAACCCCGAAACAACCAAACTCCGCGCGCCCTCGCTAAAACGTACGACG310.11121475209873No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACATACAACA300.10762717945038387No Hit
CTAACCCCGAAACAACCAAACTCCGCACACCCTCACTAAAACGTACGACG280.10045203415369162No Hit
CTAACCCCGAAACAACTAAACTCCGCACGCCCTCGCTAAAACGTACGACG280.10045203415369162No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTT100.0063269986150.36313145
GTCTCCT151.0529002E-4150.36311145
GTCTCTT27100.0145.0921145
ATCCCGA100.0070593804144.96414
CGACACA253.6217898E-8144.96418
AATCCCG100.0070593804144.96413
GAAGCAA100.0070593804144.96419
CGAAGCA100.0070593804144.96418
CCGCAAC100.0070593804144.96417
GACACAA253.6217898E-8144.96419
CCCGCAA100.0070593804144.96416
CCCCGAC253.6217898E-8144.96415
CTAATCC100.0070593804144.96411
CCCGACA253.6217898E-8144.96416
CCCGAAG100.0070593804144.96416
TCCCGAA100.0070593804144.96415
CCGACAC253.6217898E-8144.96417
CTACCCC100.0070593804144.96411
AGCCCCG151.2185064E-4144.964083
CTCCCCC151.2185064E-4144.964081
Produced by FastQC (version 0.11.5)